000078748 001__ 78748
000078748 005__ 20240118091946.0
000078748 0247_ $$2doi$$a10.1007/s13105-018-0616-2
000078748 0248_ $$2sideral$$a105340
000078748 037__ $$aART-2018-105340
000078748 041__ $$aeng
000078748 100__ $$0(orcid)0000-0002-8100-5596$$aMartínez-Beamonte, R.$$uUniversidad de Zaragoza
000078748 245__ $$aHepatic subcellular distribution of squalene changes according to the experimental setting
000078748 260__ $$c2018
000078748 5060_ $$aAccess copy available to the general public$$fUnrestricted
000078748 5203_ $$aSqualene is the main unsaponifiable component of virgin olive oil, the main source of dietary fat in Mediterranean diet, traditionally associated with a less frequency of cardiovascular diseases. In this study, two experimental approaches were used. In the first, New Zealand rabbits fed for 4 weeks with a chow diet enriched in 1% sunflower oil for the control group, and in 1% of sunflower oil and 0.5% squalene for the squalene group. In the second, APOE KO mice received either Western diet or Western diet enriched in 0.5% squalene for 11 weeks. In both studies, liver samples were obtained and analyzed for their squalene content by gas chromatography-mass spectrometry. Hepatic distribution of squalene was also characterized in isolated subcellular organelles. Our results show that dietary squalene accumulates in the liver and a differential distribution according to studied model. In this regard, rabbits accumulated in cytoplasm within small size vesicles, whose size was not big enough to be considered lipid droplets, rough endoplasmic reticulum, and nuclear and plasma membranes. On the contrary, mice accumulated in large lipid droplets, and smooth reticulum fractions in addition to nuclear and plasma membranes. These results show that the squalene cellular localization may change according to experimental setting and be a starting point to characterize the mechanisms involved in the protective action of dietary squalene in several pathologies.
000078748 536__ $$9info:eu-repo/grantAgreement/ES/MINECO/SAF2016-75441-R$$9info:eu-repo/grantAgreement/ES/MINECO/SAF2013-41651-R$$9info:eu-repo/grantAgreement/ES/ISCIII/CB06-03-1012$$9info:eu-repo/grantAgreement/ES/DGA-FSE/B69
000078748 540__ $$9info:eu-repo/semantics/openAccess$$aAll rights reserved$$uhttp://www.europeana.eu/rights/rr-f/
000078748 590__ $$a2.523$$b2018
000078748 591__ $$aPHYSIOLOGY$$b38 / 81 = 0.469$$c2018$$dQ2$$eT2
000078748 591__ $$aBIOCHEMISTRY & MOLECULAR BIOLOGY$$b169 / 294 = 0.575$$c2018$$dQ3$$eT2
000078748 592__ $$a0.872$$b2018
000078748 593__ $$aBiochemistry$$c2018$$dQ2
000078748 593__ $$aPhysiology$$c2018$$dQ2
000078748 593__ $$aMedicine (miscellaneous)$$c2018$$dQ2
000078748 655_4 $$ainfo:eu-repo/semantics/article$$vinfo:eu-repo/semantics/acceptedVersion
000078748 700__ $$aAlda, O.
000078748 700__ $$0(orcid)0000-0002-5407-3876$$aSanclemente, T.
000078748 700__ $$aFelices, M.J.
000078748 700__ $$aEscusol, S.
000078748 700__ $$0(orcid)0000-0003-1197-4276$$aArnal, C.$$uUniversidad de Zaragoza
000078748 700__ $$0(orcid)0000-0002-4665-9674$$aHerrera-Marcos, L.V.$$uUniversidad de Zaragoza
000078748 700__ $$aGascón, S.$$uUniversidad de Zaragoza
000078748 700__ $$0(orcid)0000-0002-5841-0462$$aSurra, J.C.$$uUniversidad de Zaragoza
000078748 700__ $$0(orcid)0000-0002-8251-8457$$aOsada, J.$$uUniversidad de Zaragoza
000078748 700__ $$0(orcid)0000-0002-3595-7668$$aRodríguez-Yoldi, M.J.$$uUniversidad de Zaragoza
000078748 7102_ $$12008$$2640$$aUniversidad de Zaragoza$$bDpto. Produc.Animal Cienc.Ali.$$cÁrea Nutrición Bromatología
000078748 7102_ $$11009$$2773$$aUniversidad de Zaragoza$$bDpto. Patología Animal$$cÁrea Sanidad Animal
000078748 7102_ $$11005$$2410$$aUniversidad de Zaragoza$$bDpto. Farmacología y Fisiolog.$$cÁrea Fisiología
000078748 7102_ $$12008$$2700$$aUniversidad de Zaragoza$$bDpto. Produc.Animal Cienc.Ali.$$cÁrea Producción Animal
000078748 7102_ $$11009$$2X$$aUniversidad de Zaragoza$$bDpto. Patología Animal$$cÁrea Técnica. Lab. y Talleres
000078748 7102_ $$11002$$2060$$aUniversidad de Zaragoza$$bDpto. Bioq.Biolog.Mol. Celular$$cÁrea Bioquímica y Biolog.Mole.
000078748 773__ $$g74, 4 (2018), 531-538$$pJ. physiol. biochem.$$tJournal of Physiology and Biochemistry$$x1138-7548
000078748 8564_ $$s252925$$uhttps://zaguan.unizar.es/record/78748/files/texto_completo.pdf$$yPostprint
000078748 8564_ $$s60750$$uhttps://zaguan.unizar.es/record/78748/files/texto_completo.jpg?subformat=icon$$xicon$$yPostprint
000078748 909CO $$ooai:zaguan.unizar.es:78748$$particulos$$pdriver
000078748 951__ $$a2024-01-18-09:10:35
000078748 980__ $$aARTICLE