000078749 001__ 78749
000078749 005__ 20201113085627.0
000078749 0247_ $$2doi$$a10.1016/j.jmbbm.2018.04.007
000078749 0248_ $$2sideral$$a105928
000078749 037__ $$aART-2018-105928
000078749 041__ $$aeng
000078749 100__ $$0(orcid)0000-0002-3796-4482$$aDel Amo, C.$$uUniversidad de Zaragoza
000078749 245__ $$aMatrix architecture plays a pivotal role in 3D osteoblast migration: The effect of interstitial fluid flow
000078749 260__ $$c2018
000078749 5060_ $$aAccess copy available to the general public$$fUnrestricted
000078749 5203_ $$aOsteoblast migration is a crucial process in bone regeneration, which is strongly regulated by interstitial fluid flow. However, the exact role that such flow exerts on osteoblast migration is still unclear. To deepen the understanding of this phenomenon, we cultured human osteoblasts on 3D microfluidic devices under different fluid flow regimes. Our results show that a slow fluid flow rate by itself is not able to alter the 3D migratory patterns of osteoblasts in collagen-based gels but that at higher fluid flow rates (increased flow velocity) may indirectly influence cell movement by altering the collagen microstructure. In fact, we observed that high fluid flow rates (1 µl/min) are able to alter the collagen matrix architecture and to indirectly modulate the migration pattern. However, when these collagen scaffolds were crosslinked with a chemical crosslinker, specifically, transglutaminase II, we did not find significant alterations in the scaffold architecture or in osteoblast movement. Therefore, our data suggest that high interstitial fluid flow rates can regulate osteoblast migration by means of modifying the orientation of collagen fibers. Together, these results highlight the crucial role of the matrix architecture in 3D osteoblast migration. In addition, we show that interstitial fluid flow in conjunction with the matrix architecture regulates the osteoblast morphology in 3D.
000078749 536__ $$9info:eu-repo/grantAgreement/ES/MEC/FPU13-03194$$9This project has received funding from the European Union’s Horizon 2020 research and innovation program under grant agreement No H2020 737543-IMAGO$$9info:eu-repo/grantAgreement/EC/H2020/737543/EU/Image Analysis Online Services for in-vitro experiments/IMAGO$$9info:eu-repo/grantAgreement/EUR/ERC/INSILICO-CELL-ERC-2012-StG-306571$$9info:eu-repo/grantAgreement/ES/DGA/C126-2015$$9info:eu-repo/grantAgreement/ES/MINECO/DPI2015-64221-C2-1-R
000078749 540__ $$9info:eu-repo/semantics/openAccess$$aby-nc-nd$$uhttp://creativecommons.org/licenses/by-nc-nd/3.0/es/
000078749 590__ $$a3.485$$b2018
000078749 591__ $$aENGINEERING, BIOMEDICAL$$b18 / 80 = 0.225$$c2018$$dQ1$$eT1
000078749 591__ $$aMATERIALS SCIENCE, BIOMATERIALS$$b13 / 32 = 0.406$$c2018$$dQ2$$eT2
000078749 592__ $$a1.037$$b2018
000078749 593__ $$aBiomaterials$$c2018$$dQ1
000078749 593__ $$aMechanics of Materials$$c2018$$dQ1
000078749 593__ $$aBiomedical Engineering$$c2018$$dQ1
000078749 655_4 $$ainfo:eu-repo/semantics/article$$vinfo:eu-repo/semantics/acceptedVersion
000078749 700__ $$aOlivares, V.$$uUniversidad de Zaragoza
000078749 700__ $$0(orcid)0000-0002-8656-7846$$aCóndor, M.$$uUniversidad de Zaragoza
000078749 700__ $$aBlanco, A.
000078749 700__ $$0(orcid)0000-0001-7316-0003$$aSantolaria, J.$$uUniversidad de Zaragoza
000078749 700__ $$0(orcid)0000-0002-0174-789X$$aAsín, J.$$uUniversidad de Zaragoza
000078749 700__ $$0(orcid)0000-0002-3784-1140$$aBorau, C.$$uUniversidad de Zaragoza
000078749 700__ $$0(orcid)0000-0002-9864-7683$$aGarcía-Aznar, J.M.$$uUniversidad de Zaragoza
000078749 7102_ $$12007$$2265$$aUniversidad de Zaragoza$$bDpto. Métodos Estadísticos$$cÁrea Estadís. Investig. Opera.
000078749 7102_ $$15002$$2515$$aUniversidad de Zaragoza$$bDpto. Ingeniería Diseño Fabri.$$cÁrea Ing. Procesos Fabricación
000078749 7102_ $$15004$$2605$$aUniversidad de Zaragoza$$bDpto. Ingeniería Mecánica$$cÁrea Mec.Med.Cont. y Teor.Est.
000078749 773__ $$g83 (2018), 52-62$$pJ. mech. behav. boomed. mater.$$tJournal of the Mechanical Behavior of Biomedical Materials$$x1751-6161
000078749 8564_ $$s1371582$$uhttps://zaguan.unizar.es/record/78749/files/texto_completo.pdf$$yPostprint
000078749 8564_ $$s88084$$uhttps://zaguan.unizar.es/record/78749/files/texto_completo.jpg?subformat=icon$$xicon$$yPostprint
000078749 909CO $$ooai:zaguan.unizar.es:78749$$particulos$$pdriver
000078749 951__ $$a2020-11-13-08:47:07
000078749 980__ $$aARTICLE