78749 20201113085627.0 doi 10.1016/j.jmbbm.2018.04.007 sideral 105928 ART-2018-105928 eng (orcid)0000-0002-3796-4482 Del Amo, C. Universidad de Zaragoza Matrix architecture plays a pivotal role in 3D osteoblast migration: The effect of interstitial fluid flow 2018 Access copy available to the general public Unrestricted Osteoblast migration is a crucial process in bone regeneration, which is strongly regulated by interstitial fluid flow. However, the exact role that such flow exerts on osteoblast migration is still unclear. To deepen the understanding of this phenomenon, we cultured human osteoblasts on 3D microfluidic devices under different fluid flow regimes. Our results show that a slow fluid flow rate by itself is not able to alter the 3D migratory patterns of osteoblasts in collagen-based gels but that at higher fluid flow rates (increased flow velocity) may indirectly influence cell movement by altering the collagen microstructure. In fact, we observed that high fluid flow rates (1 µl/min) are able to alter the collagen matrix architecture and to indirectly modulate the migration pattern. However, when these collagen scaffolds were crosslinked with a chemical crosslinker, specifically, transglutaminase II, we did not find significant alterations in the scaffold architecture or in osteoblast movement. Therefore, our data suggest that high interstitial fluid flow rates can regulate osteoblast migration by means of modifying the orientation of collagen fibers. Together, these results highlight the crucial role of the matrix architecture in 3D osteoblast migration. In addition, we show that interstitial fluid flow in conjunction with the matrix architecture regulates the osteoblast morphology in 3D. info:eu-repo/grantAgreement/ES/MEC/FPU13-03194 This project has received funding from the European Union’s Horizon 2020 research and innovation program under grant agreement No H2020 737543-IMAGO info:eu-repo/grantAgreement/EC/H2020/737543/EU/Image Analysis Online Services for in-vitro experiments/IMAGO info:eu-repo/grantAgreement/EUR/ERC/INSILICO-CELL-ERC-2012-StG-306571 info:eu-repo/grantAgreement/ES/DGA/C126-2015 info:eu-repo/grantAgreement/ES/MINECO/DPI2015-64221-C2-1-R info:eu-repo/semantics/openAccess by-nc-nd http://creativecommons.org/licenses/by-nc-nd/3.0/es/ 3.485 2018 ENGINEERING, BIOMEDICAL 18 / 80 = 0.225 2018 Q1 T1 MATERIALS SCIENCE, BIOMATERIALS 13 / 32 = 0.406 2018 Q2 T2 1.037 2018 Biomaterials 2018 Q1 Mechanics of Materials 2018 Q1 Biomedical Engineering 2018 Q1 info:eu-repo/semantics/article info:eu-repo/semantics/acceptedVersion Olivares, V. Universidad de Zaragoza (orcid)0000-0002-8656-7846 Cóndor, M. Universidad de Zaragoza Blanco, A. (orcid)0000-0001-7316-0003 Santolaria, J. Universidad de Zaragoza (orcid)0000-0002-0174-789X Asín, J. Universidad de Zaragoza (orcid)0000-0002-3784-1140 Borau, C. Universidad de Zaragoza (orcid)0000-0002-9864-7683 García-Aznar, J.M. Universidad de Zaragoza 2007 265 Universidad de Zaragoza Dpto. Métodos Estadísticos Área Estadís. Investig. Opera. 5002 515 Universidad de Zaragoza Dpto. Ingeniería Diseño Fabri. Área Ing. Procesos Fabricación 5004 605 Universidad de Zaragoza Dpto. Ingeniería Mecánica Área Mec.Med.Cont. y Teor.Est. 83 (2018), 52-62 J. mech. behav. boomed. mater. Journal of the Mechanical Behavior of Biomedical Materials 1751-6161 1371582 http://zaguan.unizar.es/record/78749/files/texto_completo.pdf Postprint 88084 http://zaguan.unizar.es/record/78749/files/texto_completo.jpg?subformat=icon icon Postprint oai:zaguan.unizar.es:78749 articulos driver 2020-11-13-08:47:07 ARTICLE