Degradation of extracellular matrix regulates osteoblast migration: A microfluidic-based study
Financiación FP7 / Fp7 Funds
Resumen: Bone regeneration is strongly dependent on the capacity of cells to move in a 3D microenvironment, where a large cascade of signals is activated. To improve the understanding of this complex process and to advance in the knowledge of the role of each specific signal, it is fundamental to analyze the impact of each factor independently. Microfluidic-based cell culture is an appropriate technology to achieve this objective, because it allows recreating realistic 3D local microenvironments by taking into account the extracellular matrix, cells and chemical gradients in an independent or combined scenario. The main aim of this work is to analyze the impact of extracellular matrix properties and growth factor gradients on 3D osteoblast movement, as well as the role of cell matrix degradation. For that, we used collagen-based hydrogels, with and without crosslinkers, under different chemical gradients, and eventually inhibiting metalloproteinases to tweak matrix degradation. We found that osteoblast''s 3D migratory patterns were affected by the hydrogel properties and the PDGF-BB gradient, although the strongest regulatory factor was determined by the ability of cells to remodel the matrix.
Idioma: Inglés
DOI: 10.1016/j.bone.2017.10.025
Año: 2018
Publicado en: BONE 107 (2018), 10-17
ISSN: 8756-3282

Factor impacto JCR: 4.36 (2018)
Categ. JCR: ENDOCRINOLOGY & METABOLISM rank: 31 / 145 = 0.214 (2018) - Q1 - T1
Factor impacto SCIMAGO: 1.609 - Endocrinology, Diabetes and Metabolism (Q1) - Physiology (Q1) - Histology (Q1)

Financiación: info:eu-repo/grantAgreement/EC/FP7/306571/EU/Predictive modelling and simulation in mechano-chemo-biology: a computer multi-approach/INSILICO-CELL
Financiación: info:eu-repo/grantAgreement/ES/MINECO/DPI2015-64221-C2-1-R
Tipo y forma: Artículo (PostPrint)
Área (Departamento): Área Mec.Med.Cont. y Teor.Est. (Dpto. Ingeniería Mecánica)

Creative Commons Debe reconocer adecuadamente la autoría, proporcionar un enlace a la licencia e indicar si se han realizado cambios. Puede hacerlo de cualquier manera razonable, pero no de una manera que sugiera que tiene el apoyo del licenciador o lo recibe por el uso que hace. No puede utilizar el material para una finalidad comercial. Si remezcla, transforma o crea a partir del material, no puede difundir el material modificado.


Exportado de SIDERAL (2020-01-17-22:10:53)


Visitas y descargas

Este artículo se encuentra en las siguientes colecciones:
Artículos



 Registro creado el 2019-03-29, última modificación el 2020-01-17


Postprint:
 PDF
Valore este documento:

Rate this document:
1
2
3
 
(Sin ninguna reseña)