78767 20201009175923.0 doi 10.1039/c7nr06020c sideral 104184 ART-2018-104184 eng Marín-Barba, M. Unravelling the mechanisms that determine the uptake and metabolism of magnetic single and multicore nanoparticles in a: Xenopus laevis model 2018 Access copy available to the general public Unrestricted Multicore superparamagnetic nanoparticles have been proposed as ideal tools for some biomedical applications because of their high magnetic moment per particle, high specific surface area and long term colloidal stability. Through controlled aggregation and packing of magnetic cores it is possible to obtain not only single-core but also multicore and hollow spheres with internal voids. In this work, we compare toxicological properties of single and multicore nanoparticles. Both types of particles showed moderate in vitro toxicity (MTT assay) tested in Hep G2 (human hepatocellular carcinoma) and Caco-2 (human colorectal adenocarcinoma) cells. The influence of surface chemistry in their biological behavior was also studied after functionalization with O, O'-bis(2-aminoethyl) PEG (2000 Da). For the first time, these nanoparticles were evaluated in a Xenopus laevis model studying their whole organism toxicity and their impact upon iron metabolism. The degree of activation of the metabolic pathway depends on the size and surface charge of the nanoparticles which determine their uptake. The results also highlight the potential of Xenopus laevis model bridging the gap between in vitro cell-based assays and rodent models for toxicity assessment to develop effective nanoparticles for biomedical applications. info:eu-repo/grantAgreement/ES/MINECO/MAT2014-52069-R This project has received funding from the European Union’s Horizon 2020 research and innovation program under grant agreement No H2020 705089-MIR-CHROM-C info:eu-repo/grantAgreement/EC/H2020/705089/EU/Investigating the microRNA-chromatin remodelling circuitry in cardiac development/MIR-CHROM-C info:eu-repo/grantAgreement/EC/FP7/612338/EU/DNA-TRAP Delivery of Nucleic Acid-Based Therapeutics for the TReatment of Antibiotic-Resistant Pathogens/DNA-TRAP info:eu-repo/grantAgreement/EC/FP7/607142/EU/European Initial Training Network on Developmental and Computational Biology/DEVCOM info:eu-repo/grantAgreement/EC/FP7/604448/EU/Nanometrology Standardization Methods for Magnetic Nanoparticles/NANOMAG info:eu-repo/grantAgreement/ES/MINECO/RYC-2014-15512 info:eu-repo/semantics/openAccess All rights reserved http://www.europeana.eu/rights/rr-f/ 6.97 2018 MATERIALS SCIENCE, MULTIDISCIPLINARY 41 / 293 = 0.14 2018 Q1 T1 NANOSCIENCE & NANOTECHNOLOGY 20 / 94 = 0.213 2018 Q1 T1 CHEMISTRY, MULTIDISCIPLINARY 26 / 172 = 0.151 2018 Q1 T1 PHYSICS, APPLIED 18 / 148 = 0.122 2018 Q1 T1 2.396 2018 Nanoscience and Nanotechnology 2018 Q1 Materials Science (miscellaneous) 2018 Q1 info:eu-repo/semantics/article info:eu-repo/semantics/acceptedVersion Gavilán, H. (orcid)0000-0003-2366-3598 Gutiérrez, L. Universidad de Zaragoza Lozano-Velasco, E. Rodríguez-Ramiro, I. Wheeler, G.N. Morris, C.J. Morales, M.P. Ruiz, A. 2009 750 Universidad de Zaragoza Dpto. Química Analítica Área Química Analítica 10, 2 (2018), 690-704 Nanoscale Nanoscale 2040-3364 700594 http://zaguan.unizar.es/record/78767/files/texto_completo.pdf Postprint 47396 http://zaguan.unizar.es/record/78767/files/texto_completo.jpg?subformat=icon icon Postprint oai:zaguan.unizar.es:78767 articulos driver 2020-10-09-17:47:08 ARTICLE