000078820 001__ 78820
000078820 005__ 20230210140618.0
000078820 0247_ $$2doi$$a10.1038/s41598-019-39433-6
000078820 0248_ $$2sideral$$a111134
000078820 037__ $$aART-2019-111134
000078820 041__ $$aeng
000078820 100__ $$0(orcid)0000-0003-2848-170X$$aLópez-Alonso, B.$$uUniversidad de Zaragoza
000078820 245__ $$aHistopathological and Ultrastructural Changes after Electroporation in Pig Liver Using Parallel-Plate Electrodes and High-Performance Generator
000078820 260__ $$c2019
000078820 5060_ $$aAccess copy available to the general public$$fUnrestricted
000078820 5203_ $$aIrreversible electroporation (IRE) has gained attention as a new non-thermal therapy for ablation with important benefits in terms of homogeneous treatment and fast recovery. In this study, a new concept of high voltage generator is used, enabling irreversible electroporation treatment in large tissue volume using parallel plates. Unlike currently available generators, the proposed versatile structure enables delivering high-voltage high-current pulses. To obtain homogeneous results, 3-cm parallel-plates electrodes have also been designed and implemented. IRE ablation was performed on six female pigs at 2000¿V/cm electric field, and the results were analysed after sacrifice three hours, three days and seven days after ablation. Histopathological and ultrastructural studies, including transmission and scanning electron microscopy, were carried out. The developed high-voltage generator has proved to be effective for homogeneous IRE treatment using parallel plates. The destruction of the membrane of the hepatocytes and the alterations of the membranes of the cellular organelles seem incompatible with cell death by apoptosis. Although endothelial cells also die with electroporation, the maintenance of vascular scaffold allows repairing processes to begin from the third day after IRE as long as the blood flow has not been interrupted. This study has opened new direction for IRE using high performance generators and highlighted the importance of taking into account ultrastructural changes after IRE by using electron microscopy analysis.
000078820 536__ $$9info:eu-repo/grantAgreement/ES/DGA/FSE$$9info:eu-repo/grantAgreement/ES/MINECO/TEC2016-78358-R
000078820 540__ $$9info:eu-repo/semantics/openAccess$$aby$$uhttp://creativecommons.org/licenses/by/3.0/es/
000078820 590__ $$a3.998$$b2019
000078820 591__ $$aMULTIDISCIPLINARY SCIENCES$$b17 / 71 = 0.239$$c2019$$dQ1$$eT1
000078820 592__ $$a1.341$$b2019
000078820 593__ $$aMultidisciplinary$$c2019$$dQ1
000078820 655_4 $$ainfo:eu-repo/semantics/article$$vinfo:eu-repo/semantics/publishedVersion
000078820 700__ $$aHernáez, A.
000078820 700__ $$0(orcid)0000-0001-8399-4650$$aSarnago, H.$$uUniversidad de Zaragoza
000078820 700__ $$aNaval, A.
000078820 700__ $$0(orcid)0000-0002-4437-2581$$aGüemes , A.$$uUniversidad de Zaragoza
000078820 700__ $$0(orcid)0000-0002-9951-1075$$aJunquera, C.$$uUniversidad de Zaragoza
000078820 700__ $$0(orcid)0000-0002-9655-5531$$aBurdío, J.M.$$uUniversidad de Zaragoza
000078820 700__ $$0(orcid)0000-0001-7453-2470$$aCastiella, T.$$uUniversidad de Zaragoza
000078820 700__ $$0(orcid)0000-0002-7453-1766$$aMonleón, E.$$uUniversidad de Zaragoza
000078820 700__ $$0(orcid)0000-0002-4907-8744$$aGracia-Llanes, J.$$uUniversidad de Zaragoza
000078820 700__ $$aBurdio, F.
000078820 700__ $$0(orcid)0000-0001-7264-6095$$aMejía, E.$$uUniversidad de Zaragoza
000078820 700__ $$0(orcid)0000-0002-1284-9007$$aLucía, O.$$uUniversidad de Zaragoza
000078820 7102_ $$11003$$2443$$aUniversidad de Zaragoza$$bDpto. Anatom.Histolog.Humanas$$cArea Histología
000078820 7102_ $$15008$$2785$$aUniversidad de Zaragoza$$bDpto. Ingeniería Electrón.Com.$$cÁrea Tecnología Electrónica
000078820 7102_ $$11004$$2090$$aUniversidad de Zaragoza$$bDpto. Cirugía,Ginecol.Obstetr.$$cÁrea Cirugía
000078820 7102_ $$11000$$2020$$aUniversidad de Zaragoza$$bDpto. Anat.Pat.Med.Leg.For.To.$$cArea Anatomía Patológica
000078820 773__ $$g9 (2019), 2647 [12 pp]$$pSci. rep.$$tScientific Reports$$x2045-2322
000078820 8564_ $$s1386370$$uhttps://zaguan.unizar.es/record/78820/files/texto_completo.pdf$$yVersión publicada
000078820 8564_ $$s111024$$uhttps://zaguan.unizar.es/record/78820/files/texto_completo.jpg?subformat=icon$$xicon$$yVersión publicada
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000078820 951__ $$a2023-02-10-14:00:00
000078820 980__ $$aARTICLE