78820 20230210140618.0 doi 10.1038/s41598-019-39433-6 sideral 111134 ART-2019-111134 eng López-Alonso, B. Universidad de Zaragoza (orcid)0000-0003-2848-170X Histopathological and Ultrastructural Changes after Electroporation in Pig Liver Using Parallel-Plate Electrodes and High-Performance Generator 2019 Access copy available to the general public Unrestricted Irreversible electroporation (IRE) has gained attention as a new non-thermal therapy for ablation with important benefits in terms of homogeneous treatment and fast recovery. In this study, a new concept of high voltage generator is used, enabling irreversible electroporation treatment in large tissue volume using parallel plates. Unlike currently available generators, the proposed versatile structure enables delivering high-voltage high-current pulses. To obtain homogeneous results, 3-cm parallel-plates electrodes have also been designed and implemented. IRE ablation was performed on six female pigs at 2000¿V/cm electric field, and the results were analysed after sacrifice three hours, three days and seven days after ablation. Histopathological and ultrastructural studies, including transmission and scanning electron microscopy, were carried out. The developed high-voltage generator has proved to be effective for homogeneous IRE treatment using parallel plates. The destruction of the membrane of the hepatocytes and the alterations of the membranes of the cellular organelles seem incompatible with cell death by apoptosis. Although endothelial cells also die with electroporation, the maintenance of vascular scaffold allows repairing processes to begin from the third day after IRE as long as the blood flow has not been interrupted. This study has opened new direction for IRE using high performance generators and highlighted the importance of taking into account ultrastructural changes after IRE by using electron microscopy analysis. info:eu-repo/grantAgreement/ES/DGA/FSE info:eu-repo/grantAgreement/ES/MINECO/TEC2016-78358-R info:eu-repo/semantics/openAccess by http://creativecommons.org/licenses/by/3.0/es/ 3.998 2019 MULTIDISCIPLINARY SCIENCES 17 / 71 = 0.239 2019 Q1 T1 1.341 2019 Multidisciplinary 2019 Q1 info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion Hernáez, A. Sarnago, H. Universidad de Zaragoza (orcid)0000-0001-8399-4650 Naval, A. Güemes , A. Universidad de Zaragoza (orcid)0000-0002-4437-2581 Junquera, C. Universidad de Zaragoza (orcid)0000-0002-9951-1075 Burdío, J.M. Universidad de Zaragoza (orcid)0000-0002-9655-5531 Castiella, T. Universidad de Zaragoza (orcid)0000-0001-7453-2470 Monleón, E. Universidad de Zaragoza (orcid)0000-0002-7453-1766 Gracia-Llanes, J. Universidad de Zaragoza (orcid)0000-0002-4907-8744 Burdio, F. Mejía, E. Universidad de Zaragoza (orcid)0000-0001-7264-6095 Lucía, O. Universidad de Zaragoza (orcid)0000-0002-1284-9007 1003 443 Universidad de Zaragoza Dpto. Anatom.Histolog.Humanas Area Histología 5008 785 Universidad de Zaragoza Dpto. Ingeniería Electrón.Com. Área Tecnología Electrónica 1004 090 Universidad de Zaragoza Dpto. Cirugía,Ginecol.Obstetr. Área Cirugía 1000 020 Universidad de Zaragoza Dpto. Anat.Pat.Med.Leg.For.To. Area Anatomía Patológica 9 (2019), 2647 [12 pp] Sci. rep. Scientific Reports 2045-2322 1386370 http://zaguan.unizar.es/record/78820/files/texto_completo.pdf Versión publicada 111024 http://zaguan.unizar.es/record/78820/files/texto_completo.jpg?subformat=icon icon Versión publicada oai:zaguan.unizar.es:78820 articulos driver 2023-02-10-14:00:00 ARTICLE