78862 20211216131132.0 doi 10.1371/journal.pone.0212136 sideral 111236 ART-2019-111236 eng Alcolea, P.J. IL12 p35 and p40 subunit genes administered as pPAL plasmid constructs do not improve protection of pPAL-LACK vaccine against canine leishmaniasis 2019 Access copy available to the general public Unrestricted Leishmania infantum causes zoonotic visceral leishmaniasis (ZVL) in the Mediterranean basin and South America. The parasite has been shown to co-infect HIV patients and an outbreak in central Spain was reported in the last decade. Therfore, ZVL is a public health problem, dogs being the parasite’s reservoir. We have developed a DNA vaccine based on the L. infantum activated protein kinase A receptor (LACK) using different plasmid vectors and vaccinia virus strains as vehicles. Recently, we have generated an antibiotic resistance marker-free plasmid vector called pPAL. Homologous pPAL-LACK prime-boost vaccination protects Beagle dogs as well as a heterologous plasmid-virus regime. For both reasons, pPAL improves safety. IL12 was described to trigger Th1 response through IFN-¿ production in infected dogs, being a good candidate for cytokine therapy in conventional treatment-unresponsive dogs. Herein, we report a complete protection study in dogs through inoculation of genes encoding for the p35 and p40 subunits which compose canine IL12 in combination with the LACK gene. A homologous plasmid-plasmid regime using independent pPAL constructs for each gene was inoculated in a 15-day interval. The infectious challenge using L. infantum promastigotes was successful. The outcome was pPAL-LACK vaccine protection suppression by IL12 administration. The important implications of this finding are discussed in the manuscript. info:eu-repo/semantics/openAccess by http://creativecommons.org/licenses/by/3.0/es/ 2.74 2019 MULTIDISCIPLINARY SCIENCES 27 / 71 = 0.38 2019 Q2 T2 1.023 2019 Multidisciplinary 2019 Q1 info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion Alonso, A. Esteban, A. (orcid)0000-0002-8282-5975 Peris, P. Universidad de Zaragoza (orcid)0000-0001-5750-3643 Cortés, A. Universidad de Zaragoza (orcid)0000-0002-2218-5072 Castillo, J.A. Universidad de Zaragoza (orcid)0000-0002-2048-4749 Larraga, V. 1009 773 Universidad de Zaragoza Dpto. Patología Animal Área Sanidad Animal 1009 617 Universidad de Zaragoza Dpto. Patología Animal Área Medicina y Cirugía Animal 14, 2 (2019), e0212136[18 pp] PLoS One PloS one 1932-6203 475928 http://zaguan.unizar.es/record/78862/files/texto_completo.pdf Versión publicada 91848 http://zaguan.unizar.es/record/78862/files/texto_completo.jpg?subformat=icon icon Versión publicada oai:zaguan.unizar.es:78862 articulos driver 2021-12-16-13:01:55 ARTICLE