78913 20200113145614.0 doi 10.1016/j.jpba.2018.04.019 sideral 106400 ART-2018-106400 eng Pires, F. A rapid magnetic particle-based enzyme immunoassay for human cytomegalovirus glycoprotein B quantification 2018 Access copy available to the general public Unrestricted Human cytomegalovirus (HCMV) is a herpes virus that can cause severe infections. Still, the available methods for its diagnostic have the main disadvantage of requiring long time to be performed. In this work, a simple magnetic particle-based enzyme immunoassay (mpEIA) for the quantification of glycoprotein B of Human cytomegalovirus (gB-HCMV) in urine samples is proposed. The immunosensor scheme is based on the analyte protein gB-HCMV sandwiched between a primary monoclonal antibody, (MBs-PrG-mAb1), and a secondary anti-gB-HCMV antibody labelled with Horseradish peroxidase (Ab2-HRP) to allow spectrophotometric detection. The mpEIA analytical performance was tested in urine samples, showing a linear dependence between gB-HCMV concentration and the absorbance signal at 450 nm in a range of concentrations from 90 to 700 pg mL-1. The calculated detection limits for gB-HCMV were 90 ± 2 pg mL-1 and the RSD was about 6.7% in urine samples. The immunosensor showed good selectivity against other viruses from Herpesviridae family, namely varicella zoster and Epstein Barr viruses. The recoveries of spiked human urine samples at 0.30–0.50 ng mL-1 concentration levels of gB-HCMV ranged between 91 to 105%. The proposed mpEIA method was validated following the guidelines of the European Medicines Agency (EMEA-2014), and allows rapid, successful and easy quantification of gB-HCMV in urine samples. info:eu-repo/grantAgreement/ES/FEDER/POCI-01-0145-007491 info:eu-repo/grantAgreement/ES/MINECO-FEDER/MUSSEL-RTC-2015-4077-2 info:eu-repo/grantAgreement/ES/MINECO-FEDER/TEC20013-40561-P info:eu-repo/semantics/openAccess by-nc-nd http://creativecommons.org/licenses/by-nc-nd/3.0/es/ 2.983 2018 PHARMACOLOGY & PHARMACY 105 / 266 = 0.395 2018 Q2 T2 CHEMISTRY, ANALYTICAL 24 / 84 = 0.286 2018 Q2 T1 0.786 2018 Analytical Chemistry 2018 Q1 Clinical Biochemistry 2018 Q1 Spectroscopy 2018 Q1 Pharmaceutical Science 2018 Q1 Drug Discovery 2018 Q1 info:eu-repo/semantics/article info:eu-repo/semantics/acceptedVersion Arcos-Martinez, M.J. Dias-Cabral, A.C. (orcid)0000-0003-4599-796X Vidal, J.C. Universidad de Zaragoza (orcid)0000-0002-4070-8607 Castillo, J.R. Universidad de Zaragoza 2009 750 Universidad de Zaragoza Dpto. Química Analítica Área Química Analítica 156 (2018), 372-378 J. pharm. biomed. anal. Journal of Pharmaceutical and Biomedical Analysis 0731-7085 412528 http://zaguan.unizar.es/record/78913/files/texto_completo.pdf Postprint 45280 http://zaguan.unizar.es/record/78913/files/texto_completo.jpg?subformat=icon icon Postprint oai:zaguan.unizar.es:78913 articulos driver 2020-01-13-14:52:23 ARTICLE