000078921 001__ 78921
000078921 005__ 20230921135425.0
000078921 0247_ $$2doi$$a10.1038/s41598-019-42529-8
000078921 0248_ $$2sideral$$a111405
000078921 037__ $$aART-2019-111405
000078921 041__ $$aeng
000078921 100__ $$0(orcid)0000-0003-3660-8651$$aVirumbrales-Muñoz, M.
000078921 245__ $$aEnabling cell recovery from 3D cell culture microfluidic devices for tumour microenvironment biomarker profiling
000078921 260__ $$c2019
000078921 5060_ $$aAccess copy available to the general public$$fUnrestricted
000078921 5203_ $$aThe tumour microenvironment (TME) has recently drawn much attention due to its profound impact on tumour development, drug resistance and patient outcome. There is an increasing interest in new therapies that target the TME. Nonetheless, most established in vitro models fail to include essential cues of the TME. Microfluidics can be used to reproduce the TME in vitro and hence provide valuable insight on tumour evolution and drug sensitivity. However, microfluidics remains far from well-established mainstream molecular and cell biology methods. Therefore, we have developed a quick and straightforward collagenase-based enzymatic method to recover cells embedded in a 3D hydrogel in a microfluidic device with no impact on cell viability. We demonstrate the validity of this method on two different cell lines in a TME microfluidic model. Cells were successfully retrieved with high viability, and we characterised the different cell death mechanisms via AMNIS image cytometry in our model.
000078921 536__ $$9info:eu-repo/grantAgreement/ES/DGA/T24-17R$$9info:eu-repo/grantAgreement/EC/H2020/766884/EU/Organ on Chip in Development/ORCHID$$9This project has received funding from the European Union’s Horizon 2020 research and innovation program under grant agreement No H2020 766884-ORCHID$$9info:eu-repo/grantAgreement/EC/H2020/778354/EU/Heart On chip based on induced pluripotent Stem cell Technology for personalized Medicine/CISTEM$$9This project has received funding from the European Union’s Horizon 2020 research and innovation program under grant agreement No H2020 778354-CISTEM$$9info:eu-repo/grantAgreement/ES/MINECO/BIO2016-79092-R$$9info:eu-repo/grantAgreement/ES/MINECO/DPI2015-65401-C3-1-R
000078921 540__ $$9info:eu-repo/semantics/openAccess$$aby$$uhttp://creativecommons.org/licenses/by/3.0/es/
000078921 590__ $$a3.998$$b2019
000078921 592__ $$a1.341$$b2019
000078921 591__ $$aMULTIDISCIPLINARY SCIENCES$$b17 / 71 = 0.239$$c2019$$dQ1$$eT1
000078921 593__ $$aMultidisciplinary$$c2019$$dQ1
000078921 655_4 $$ainfo:eu-repo/semantics/article$$vinfo:eu-repo/semantics/publishedVersion
000078921 700__ $$aAyuso, J.M.
000078921 700__ $$0(orcid)0000-0003-4970-2460$$aLacueva, A.$$uUniversidad de Zaragoza
000078921 700__ $$aRandelovic, T.
000078921 700__ $$aLivingston, M.K.
000078921 700__ $$aBeebe, D.J.
000078921 700__ $$0(orcid)0000-0003-0156-4230$$aOliván, S.$$uUniversidad de Zaragoza
000078921 700__ $$0(orcid)0000-0002-9357-143X$$aPereboom, D.$$uUniversidad de Zaragoza
000078921 700__ $$0(orcid)0000-0001-8741-6452$$aDoblare, M.$$uUniversidad de Zaragoza
000078921 700__ $$0(orcid)0000-0001-5376-4440$$aFernández, L.$$uUniversidad de Zaragoza
000078921 700__ $$0(orcid)0000-0003-2410-5678$$aOchoa, I.$$uUniversidad de Zaragoza
000078921 7102_ $$11005$$2410$$aUniversidad de Zaragoza$$bDpto. Farmacología y Fisiolog.$$cÁrea Fisiología
000078921 7102_ $$11003$$2443$$aUniversidad de Zaragoza$$bDpto. Anatom.Histolog.Humanas$$cArea Histología
000078921 7102_ $$15004$$2605$$aUniversidad de Zaragoza$$bDpto. Ingeniería Mecánica$$cÁrea Mec.Med.Cont. y Teor.Est.
000078921 773__ $$g9, 1 (2019), 6199 [14 pp]$$pSci. rep. (Nat. Publ. Group)$$tScientific reports (Nature Publishing Group)$$x2045-2322
000078921 8564_ $$s1126067$$uhttps://zaguan.unizar.es/record/78921/files/texto_completo.pdf$$yVersión publicada
000078921 8564_ $$s112077$$uhttps://zaguan.unizar.es/record/78921/files/texto_completo.jpg?subformat=icon$$xicon$$yVersión publicada
000078921 909CO $$ooai:zaguan.unizar.es:78921$$particulos$$pdriver
000078921 951__ $$a2023-09-21-13:29:04
000078921 980__ $$aARTICLE