78921 20230921135425.0 doi 10.1038/s41598-019-42529-8 sideral 111405 ART-2019-111405 eng Virumbrales-Muñoz, M. (orcid)0000-0003-3660-8651 Enabling cell recovery from 3D cell culture microfluidic devices for tumour microenvironment biomarker profiling 2019 Access copy available to the general public Unrestricted The tumour microenvironment (TME) has recently drawn much attention due to its profound impact on tumour development, drug resistance and patient outcome. There is an increasing interest in new therapies that target the TME. Nonetheless, most established in vitro models fail to include essential cues of the TME. Microfluidics can be used to reproduce the TME in vitro and hence provide valuable insight on tumour evolution and drug sensitivity. However, microfluidics remains far from well-established mainstream molecular and cell biology methods. Therefore, we have developed a quick and straightforward collagenase-based enzymatic method to recover cells embedded in a 3D hydrogel in a microfluidic device with no impact on cell viability. We demonstrate the validity of this method on two different cell lines in a TME microfluidic model. Cells were successfully retrieved with high viability, and we characterised the different cell death mechanisms via AMNIS image cytometry in our model. info:eu-repo/grantAgreement/ES/DGA/T24-17R info:eu-repo/grantAgreement/EC/H2020/766884/EU/Organ on Chip in Development/ORCHID This project has received funding from the European Union’s Horizon 2020 research and innovation program under grant agreement No H2020 766884-ORCHID info:eu-repo/grantAgreement/EC/H2020/778354/EU/Heart On chip based on induced pluripotent Stem cell Technology for personalized Medicine/CISTEM This project has received funding from the European Union’s Horizon 2020 research and innovation program under grant agreement No H2020 778354-CISTEM info:eu-repo/grantAgreement/ES/MINECO/BIO2016-79092-R info:eu-repo/grantAgreement/ES/MINECO/DPI2015-65401-C3-1-R info:eu-repo/semantics/openAccess by http://creativecommons.org/licenses/by/3.0/es/ 3.998 2019 MULTIDISCIPLINARY SCIENCES 17 / 71 = 0.239 2019 Q1 T1 1.341 2019 Multidisciplinary 2019 Q1 info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion Ayuso, J.M. Lacueva, A. Universidad de Zaragoza (orcid)0000-0003-4970-2460 Randelovic, T. Livingston, M.K. Beebe, D.J. Oliván, S. Universidad de Zaragoza (orcid)0000-0003-0156-4230 Pereboom, D. Universidad de Zaragoza (orcid)0000-0002-9357-143X Doblare, M. Universidad de Zaragoza (orcid)0000-0001-8741-6452 Fernández, L. Universidad de Zaragoza (orcid)0000-0001-5376-4440 Ochoa, I. Universidad de Zaragoza (orcid)0000-0003-2410-5678 1005 410 Universidad de Zaragoza Dpto. Farmacología y Fisiolog. Área Fisiología 1003 443 Universidad de Zaragoza Dpto. Anatom.Histolog.Humanas Area Histología 5004 605 Universidad de Zaragoza Dpto. Ingeniería Mecánica Área Mec.Med.Cont. y Teor.Est. 9, 1 (2019), 6199 [14 pp] Sci. rep. (Nat. Publ. Group) Scientific reports (Nature Publishing Group) 2045-2322 1126067 http://zaguan.unizar.es/record/78921/files/texto_completo.pdf Versión publicada 112077 http://zaguan.unizar.es/record/78921/files/texto_completo.jpg?subformat=icon icon Versión publicada oai:zaguan.unizar.es:78921 articulos driver 2023-09-21-13:29:04 ARTICLE