78930 20231023123339.0 doi 10.25107/2474-1647 sideral 111136 ART-2019-111136 eng Pañella, C. Hypersaline infusion protocol through the portal vein may focus electroporation on tumor tissue, but is it really safe? Ppreliminary results 2019 Access copy available to the general public Unrestricted Introduction: irreversible Electroporation (IRE) is highly dependent on the electrical conductivity of the tissue and the high conductivity of tumor tissue, which leads to a lower field than that in the surrounding healthy tissue. Hypersaline Infusion (HI) through the portal vein focuses IRE on scattered liver tumors, by creating a differential conductivity between the different types of tissue. The aim of this study is to determine the effects of the HI protocol on the hepatic and histological biochemical results. Methods: Ten male Sprague Dawley rats were used for HI protocol. Blood samples were collected at pre-, immediately post-, 24-hrs, 72-hrs, 1- week and 3-weeks post-HI. All the animals were sacrificed after a one-month follow-up in order to collect histological samples. Results: The mortality rate in this procedure reached 30% (3/10). Only the pH and transaminases at 24-hrs were significantly and directly linked to mortality (p=0.036 and p=0.004, respectively). The three non-surviving animals had a four-time higher AST level at 24-hrs. Natremianormalized at 24-hrs post-HI. Statistically significant differences were found in hepatic necrosis between the non-surviving (n=3) and surviving rats (n=7) (30.67 ± 10.97 vs. 2.86 ± 7.56% respectively, p=0.01). Discussion: HI through the portal system involves a significant risk of possibly lethal cytolysis and acidosis. Therefore, compensatory measures and a reduced saline overload are warranted to improve the survival rates. info:eu-repo/semantics/openAccess by-nc http://creativecommons.org/licenses/by-nc/3.0/es/ info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion Moll, X. Quesada, R. Villanueva, A. Iglesias, M. Andaluz, A. Lucía, O. Universidad de Zaragoza (orcid)0000-0002-1284-9007 Sánchez-Velázquez, P. Grande, L. Burdío, F. 5008 785 Universidad de Zaragoza Dpto. Ingeniería Electrón.Com. Área Tecnología Electrónica 4, 1 (2019), 2335 [5 pp.] Clin. surg. Clinics in Surgery 2474-1647 16326554 http://zaguan.unizar.es/record/78930/files/texto_completo.pdf Versión publicada 112954 http://zaguan.unizar.es/record/78930/files/texto_completo.jpg?subformat=icon icon Versión publicada http://www.clinicsinsurgery.com/pdfs_folder/cis-v4-id2335.pdf Texto completo de la revista oai:zaguan.unizar.es:78930 articulos driver 2023-10-23-12:20:20 ARTICLE