000078933 001__ 78933
000078933 005__ 20221027132638.0
000078933 0247_ $$2doi$$a10.1080/10826076.2018.1561465
000078933 0248_ $$2sideral$$a111261
000078933 037__ $$aART-2019-111261
000078933 041__ $$aeng
000078933 100__ $$aLapieza, M.P.
000078933 245__ $$aHPTLC coupled to ESI-Tandem MS for identifying phospholipids associated to membrane proteins in photosynthetic purple bacteria
000078933 260__ $$c2019
000078933 5060_ $$aAccess copy available to the general public$$fUnrestricted
000078933 5203_ $$aHigh-performance thin-layer chromatography (HPTLC)-densitometry was directly combined with electrospray (ESI) tandem mass spectrometry for obtaining rapid and relevant structural identification of phospholipids (PL) species associated to membrane proteins (MP), in non-sulfur, purple bacteria having photosynthetic activity. Thus, species belonging to phosphatidylcholines (PC), phosphatidylethanolamines (PE), cardiolipins (CL) and phosphatidylglycerols (PG) associated to MP were investigated in bacterial membrane extracts from Rhodobacter (Rb.) blasticus, Rhodospirillum (R.) rubrum and Rhodobaca (Rbc.) bogoriensis, as well as those which are bound to a purified MP-photosynthetic complex from Rbc. bogoriensis. PL-classes were separated using a 7-step gradient-solvent sequence with a previous acid plate preconditioning, using Automated Multiple Development. Band zones of the plate corresponding to PL classes were selected to ensure their direct transfer to ion-trap MS equipment through an elution-based interface. Under the studied conditions, ESI + -MS spectra of PC and CL mostly showed sodium adducts ([M + Na] + ) and [M-2H + 3Na] + , respectively, when recorded from the plate. The respective sodium adducts were fragmented in the ion-trap, and sodium remained as the charge of the fragment ions, thus being useful for their structural identification through MS/MS. ESI - -MS and MS/MS spectra of CL were also obtained as [M-2H] 2- , as well as those of PE and PG species as [M-H] - and [M] - , respectively. In this way, relative composition profiles of each studied PL-class by ESI-MS, and further identification of individual PL and the molecular species belonging to each of them by MS/MS were obtained.
000078933 536__ $$9info:eu-repo/grantAgreement/ES/DGA-FSE/E25-17R
000078933 540__ $$9info:eu-repo/semantics/openAccess$$aAll rights reserved$$uhttp://www.europeana.eu/rights/rr-f/
000078933 590__ $$a0.992$$b2019
000078933 591__ $$aCHEMISTRY, ANALYTICAL$$b75 / 86 = 0.872$$c2019$$dQ4$$eT3
000078933 591__ $$aBIOCHEMICAL RESEARCH METHODS$$b74 / 77 = 0.961$$c2019$$dQ4$$eT3
000078933 592__ $$a0.268$$b2019
000078933 593__ $$aPharmaceutical Science$$c2019$$dQ2
000078933 593__ $$aAnalytical Chemistry$$c2019$$dQ3
000078933 593__ $$aClinical Biochemistry$$c2019$$dQ4
000078933 593__ $$aBiochemistry$$c2019$$dQ4
000078933 655_4 $$ainfo:eu-repo/semantics/article$$vinfo:eu-repo/semantics/publishedVersion
000078933 700__ $$aJungas, C.
000078933 700__ $$0(orcid)0000-0001-7299-0187$$aSavirón, M.
000078933 700__ $$aJarne, C.
000078933 700__ $$aMembrado, L.
000078933 700__ $$0(orcid)0000-0002-4887-1652$$aVela, J.$$uUniversidad de Zaragoza
000078933 700__ $$0(orcid)0000-0003-3514-2570$$aOrduna, J.$$uUniversidad de Zaragoza
000078933 700__ $$0(orcid)0000-0003-2607-7834$$aGarriga, R.$$uUniversidad de Zaragoza
000078933 700__ $$0(orcid)0000-0002-8973-5104$$aGalbán, J.$$uUniversidad de Zaragoza
000078933 700__ $$aCebolla, V.L.
000078933 7102_ $$12009$$2750$$aUniversidad de Zaragoza$$bDpto. Química Analítica$$cÁrea Química Analítica
000078933 7102_ $$12013$$2765$$aUniversidad de Zaragoza$$bDpto. Química Orgánica$$cÁrea Química Orgánica
000078933 7102_ $$12012$$2755$$aUniversidad de Zaragoza$$bDpto. Química Física$$cÁrea Química Física
000078933 773__ $$g42, 1-2 (2019), [9 pp]$$pJ. liq. chromatogr. relat. technol.$$tJOURNAL OF LIQUID CHROMATOGRAPHY & RELATED TECHNOLOGIES$$x1082-6076
000078933 8564_ $$s1059013$$uhttps://zaguan.unizar.es/record/78933/files/texto_completo.pdf$$yVersión publicada
000078933 8564_ $$s12267$$uhttps://zaguan.unizar.es/record/78933/files/texto_completo.jpg?subformat=icon$$xicon$$yVersión publicada
000078933 909CO $$ooai:zaguan.unizar.es:78933$$particulos$$pdriver
000078933 951__ $$a2022-10-27-13:09:58
000078933 980__ $$aARTICLE