78933 20221027132638.0 doi 10.1080/10826076.2018.1561465 sideral 111261 ART-2019-111261 eng Lapieza, M.P. HPTLC coupled to ESI-Tandem MS for identifying phospholipids associated to membrane proteins in photosynthetic purple bacteria 2019 Access copy available to the general public Unrestricted High-performance thin-layer chromatography (HPTLC)-densitometry was directly combined with electrospray (ESI) tandem mass spectrometry for obtaining rapid and relevant structural identification of phospholipids (PL) species associated to membrane proteins (MP), in non-sulfur, purple bacteria having photosynthetic activity. Thus, species belonging to phosphatidylcholines (PC), phosphatidylethanolamines (PE), cardiolipins (CL) and phosphatidylglycerols (PG) associated to MP were investigated in bacterial membrane extracts from Rhodobacter (Rb.) blasticus, Rhodospirillum (R.) rubrum and Rhodobaca (Rbc.) bogoriensis, as well as those which are bound to a purified MP-photosynthetic complex from Rbc. bogoriensis.   PL-classes were separated using a 7-step gradient-solvent sequence with a previous acid plate preconditioning, using Automated Multiple Development. Band zones of the plate corresponding to PL classes were selected to ensure their direct transfer to ion-trap MS equipment through an elution-based interface.   Under the studied conditions, ESI + -MS spectra of PC and CL mostly showed sodium adducts ([M + Na] + ) and [M-2H + 3Na] + , respectively, when recorded from the plate. The respective sodium adducts were fragmented in the ion-trap, and sodium remained as the charge of the fragment ions, thus being useful for their structural identification through MS/MS. ESI - -MS and MS/MS spectra of CL were also obtained as [M-2H] 2- , as well as those of PE and PG species as [M-H] - and [M] - , respectively.   In this way, relative composition profiles of each studied PL-class by ESI-MS, and further identification of individual PL and the molecular species belonging to each of them by MS/MS were obtained. info:eu-repo/grantAgreement/ES/DGA-FSE/E25-17R info:eu-repo/semantics/openAccess All rights reserved http://www.europeana.eu/rights/rr-f/ 0.992 2019 CHEMISTRY, ANALYTICAL 75 / 86 = 0.872 2019 Q4 T3 BIOCHEMICAL RESEARCH METHODS 74 / 77 = 0.961 2019 Q4 T3 0.268 2019 Pharmaceutical Science 2019 Q2 Analytical Chemistry 2019 Q3 Clinical Biochemistry 2019 Q4 Biochemistry 2019 Q4 info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion Jungas, C. Savirón, M. (orcid)0000-0001-7299-0187 Jarne, C. Membrado, L. Vela, J. Universidad de Zaragoza (orcid)0000-0002-4887-1652 Orduna, J. Universidad de Zaragoza (orcid)0000-0003-3514-2570 Garriga, R. Universidad de Zaragoza (orcid)0000-0003-2607-7834 Galbán, J. Universidad de Zaragoza (orcid)0000-0002-8973-5104 Cebolla, V.L. 2009 750 Universidad de Zaragoza Dpto. Química Analítica Área Química Analítica 2013 765 Universidad de Zaragoza Dpto. Química Orgánica Área Química Orgánica 2012 755 Universidad de Zaragoza Dpto. Química Física Área Química Física 42, 1-2 (2019), [9 pp] J. liq. chromatogr. relat. technol. JOURNAL OF LIQUID CHROMATOGRAPHY & RELATED TECHNOLOGIES 1082-6076 1059013 http://zaguan.unizar.es/record/78933/files/texto_completo.pdf Versión publicada 12267 http://zaguan.unizar.es/record/78933/files/texto_completo.jpg?subformat=icon icon Versión publicada oai:zaguan.unizar.es:78933 articulos driver 2022-10-27-13:09:58 ARTICLE