000078944 001__ 78944
000078944 005__ 20200428120342.0
000078944 0247_ $$2doi$$a10.1111/andr.12504
000078944 0248_ $$2sideral$$a108793
000078944 037__ $$aART-2018-108793
000078944 041__ $$aeng
000078944 100__ $$aRocco, M.
000078944 245__ $$aMelatonin affects the motility and adhesiveness of in vitro capacitated boar spermatozoa via a mechanism that does not depend on intracellular ROS levels
000078944 260__ $$c2018
000078944 5060_ $$aAccess copy available to the general public$$fUnrestricted
000078944 5203_ $$aThis work sought to address the effects of melatonin during in vitro capacitation (IVC) and progesterone-induced acrosome exocytosis (IVAE) in boar spermatozoa. With this purpose, two different experiments were set. In the first one, IVC and IVAE were induced in the absence or presence of melatonin, which was added either at the start of IVC or upon triggering the IVAE with progesterone. Different parameters were evaluated, including intracellular levels of peroxides and superoxides, free cysteine radicals and distribution of specific lectins. While melatonin neither affected most capacitation-associated parameters nor IVAE, it dramatically decreased sperm motility, with a maximal effect at 5 µm. This effect was accompanied by a significant increase in the percentage of agglutinated spermatozoa, which was independent from noticeable changes in the distribution of lectins. Levels of free cysteine radicals were significantly lower in melatonin treatments than in the control after 4 h of incubation in capacitating medium. The second experiment evaluated the effects of melatonin on in vitro fertilising ability of boar spermatozoa. Spermatozoa previously subjected to IVC in the presence of 1 µm melatonin and used for in vitro fertilisation exhibited less ability to bind the zona pellucida (ZP) and higher percentages of monospermy. In conclusion, melatonin affects sperm motility and the stability of nucleoprotein structure and also modulates the ability of in vitro capacitated boar spermatozoa to bind the oocyte ZP. However, such effects do not seem to be related to either its antioxidant properties or changes in the sperm glycocalix.
000078944 536__ $$9info:eu-repo/grantAgreement/ES/MINECO/RYC-2014-15581$$9info:eu-repo/grantAgreement/ES/MINECO-FEDER/AGL2013-43328-P
000078944 540__ $$9info:eu-repo/semantics/openAccess$$aAll rights reserved$$uhttp://www.europeana.eu/rights/rr-f/
000078944 590__ $$a3.106$$b2018
000078944 591__ $$aANDROLOGY$$b1 / 7 = 0.143$$c2018$$dQ1$$eT1
000078944 592__ $$a1.123$$b2018
000078944 593__ $$aEndocrinology$$c2018$$dQ1
000078944 593__ $$aUrology$$c2018$$dQ1
000078944 593__ $$aReproductive Medicine$$c2018$$dQ1
000078944 593__ $$aEndocrinology, Diabetes and Metabolism$$c2018$$dQ1
000078944 655_4 $$ainfo:eu-repo/semantics/article$$vinfo:eu-repo/semantics/acceptedVersion
000078944 700__ $$aBetarelli, R.
000078944 700__ $$aPlacci, A.
000078944 700__ $$aFernández-Novell, J.M.
000078944 700__ $$aSpinaci, M.
000078944 700__ $$0(orcid)0000-0003-1997-4262$$aCasao, A.$$uUniversidad de Zaragoza
000078944 700__ $$0(orcid)0000-0003-1306-8517$$aMuiño-Blanco, T.$$uUniversidad de Zaragoza
000078944 700__ $$0(orcid)0000-0001-9842-6197$$aCebrián-Pérez, J.A.$$uUniversidad de Zaragoza
000078944 700__ $$aPeña, A.
000078944 700__ $$aRigau, T.
000078944 700__ $$aBonet, S.
000078944 700__ $$aCastillo-Martín, M.
000078944 700__ $$aYeste, M.
000078944 700__ $$aRodríguez-Gil, J.E.
000078944 7102_ $$11002$$2819$$aUniversidad de Zaragoza$$bDpto. Bioq.Biolog.Mol. Celular$$cÁrea Zoología
000078944 7102_ $$11002$$2060$$aUniversidad de Zaragoza$$bDpto. Bioq.Biolog.Mol. Celular$$cÁrea Bioquímica y Biolog.Mole.
000078944 773__ $$g6, 5 (2018), 720-736$$pAndrology$$tAndrology$$x2047-2919
000078944 8564_ $$s412921$$uhttps://zaguan.unizar.es/record/78944/files/texto_completo.pdf$$yPostprint
000078944 8564_ $$s118344$$uhttps://zaguan.unizar.es/record/78944/files/texto_completo.jpg?subformat=icon$$xicon$$yPostprint
000078944 909CO $$ooai:zaguan.unizar.es:78944$$particulos$$pdriver
000078944 951__ $$a2020-04-28-12:01:27
000078944 980__ $$aARTICLE