000078997 001__ 78997
000078997 005__ 20200716101453.0
000078997 0247_ $$2doi$$a10.3390/nu11040724
000078997 0248_ $$2sideral$$a111464
000078997 037__ $$aART-2019-111464
000078997 041__ $$aeng
000078997 100__ $$aAllaoui, A.
000078997 245__ $$aProtein hydrolysates from fenugreek (Trigonella foenum graecum) as nutraceutical molecules in colon cancer treatment
000078997 260__ $$c2019
000078997 5060_ $$aAccess copy available to the general public$$fUnrestricted
000078997 5203_ $$aThe application of plant extracts for therapeutic purposes has been used in traditional medicine since the plants are a source of a great variety of chemical compounds that possess biological activity. Actually, the effect of these extracts on diseases such as cancer is being widely studied. Colorectal adenocarcinoma is one of the main causes of cancer related to death and the second most prevalent carcinoma in Western countries. The aim of this work is to study the possible effect of two fenugreek (Trigonella foenum graecum) protein hydrolysates on treatment and progression of colorectal cancer. Fenugreek proteins from seeds were hydrolysed by using two enzymes separately, which are named Purafect and Esperase, and were then tested on differentiated and undifferentiated human colonic adenocarcinoma Caco2/TC7 cells. Both hydrolysates did not affect the growth of differentiated cells, while they caused a decrease in undifferentiated cell proliferation by early apoptosis and cell cycle arrest in phase G1. This was triggered by a mitochondrial membrane permeabilization, cytochrome C release to cytoplasm, and caspase-3 activation. In addition, the hydrolysates of fenugreek proteins displayed antioxidant activity since they reduce the intracellular levels of ROS. These findings suggest that fenugreek protein hydrolysates could be used as nutraceutical molecules in colorectal cancer treatment.
000078997 536__ $$9info:eu-repo/grantAgreement/ES/DGA/A32$$9info:eu-repo/grantAgreement/ES/DGA/B16-R17$$9info:eu-repo/grantAgreement/ES/ISCIII/CB06-03-1012$$9info:eu-repo/grantAgreement/ES/MINECO/SAF2016-75441-R$$9info:eu-repo/grantAgreement/EUR/SUDOE/INTERREG/Redvalue-SOE1-PI-E0123
000078997 540__ $$9info:eu-repo/semantics/openAccess$$aby$$uhttp://creativecommons.org/licenses/by/3.0/es/
000078997 590__ $$a4.546$$b2019
000078997 591__ $$aNUTRITION & DIETETICS$$b17 / 89 = 0.191$$c2019$$dQ1$$eT1
000078997 592__ $$a1.329$$b2019
000078997 593__ $$aNutrition and Dietetics$$c2019$$dQ1
000078997 593__ $$aFood Science$$c2019$$dQ1
000078997 655_4 $$ainfo:eu-repo/semantics/article$$vinfo:eu-repo/semantics/publishedVersion
000078997 700__ $$aGascón, S.$$uUniversidad de Zaragoza
000078997 700__ $$aBenomar, S.
000078997 700__ $$aQuero, J.
000078997 700__ $$0(orcid)0000-0002-8251-8457$$aOsada, J.$$uUniversidad de Zaragoza
000078997 700__ $$aNasri, M.
000078997 700__ $$0(orcid)0000-0002-3595-7668$$aRodríguez-Yoldi, M.J.$$uUniversidad de Zaragoza
000078997 700__ $$aBoualga, A.
000078997 7102_ $$11005$$2410$$aUniversidad de Zaragoza$$bDpto. Farmacología y Fisiolog.$$cÁrea Fisiología
000078997 7102_ $$11009$$2X$$aUniversidad de Zaragoza$$bDpto. Patología Animal$$cÁrea Técnica. Lab. y Talleres
000078997 7102_ $$11002$$2060$$aUniversidad de Zaragoza$$bDpto. Bioq.Biolog.Mol. Celular$$cÁrea Bioquímica y Biolog.Mole.
000078997 773__ $$g11, 4 (2019), 724 [15 pp]$$pNUTRIENTS$$tNutrients$$x2072-6643
000078997 8564_ $$s839919$$uhttps://zaguan.unizar.es/record/78997/files/texto_completo.pdf$$yVersión publicada
000078997 8564_ $$s101062$$uhttps://zaguan.unizar.es/record/78997/files/texto_completo.jpg?subformat=icon$$xicon$$yVersión publicada
000078997 909CO $$ooai:zaguan.unizar.es:78997$$particulos$$pdriver
000078997 951__ $$a2020-07-16-09:07:54
000078997 980__ $$aARTICLE