79071 20191126134629.0 doi 10.1016/j.ejpb.2018.04.016 sideral 106410 ART-2018-106410 eng Koloczek, P. Polymeric micelle-mediated delivery of half-sandwich ruthenium(II) complexes with phosphanes derived from fluoroloquinolones for lung adenocarcinoma treatment 2018 Access copy available to the general public Unrestricted Novel half-sandwich ruthenium(II) complexes with aminomethyl(diphenyl)phosphine derived from fluoroloquinolones (RuPCp, RuPSf, RuPLm, RuPNr) were being investigated as alternatives to well-established metal-based chemotherapeutics. All compounds were characterized by elemental analysis, selected spectroscopic methods (i.e., absorption and fluorescence spectroscopies, ESI-MS, NMR, circular dichroizm), X-ray diffractometry, ICP-MS, and electrochemical techniques. To overcome low solubility, serious side effects connected with systemic cytotoxicity of ruthenium complexes, and acquiring the resistance of cancer cells, polymeric nanoformulations based on Pluronic P-123 micelles loaded with selected Ru(II) complexes were prepared and characterized. Resulting micelles (RuPCp_M, RuPNr_M) enabled efficient drug accumulation inside human lung adenocarcinoma (A549 tumor cell line), proved by confocal microscopy and ICP-MS analysis, allowing cytotoxic action. Studied complexes exhibited promising cytotoxicity in vitro with IC50 values significantly lower than the reference drug – cisplatin. The fluorescence spectroscopic data (CT-DNA titration, in vitro cell staining) together with analysis of DNA fragmentation (pBR322 plasmid, comet assay) provided clear evidence for the interaction with DNA inducing apoptotic cell death. info:eu-repo/grantAgreement/ES/ISCIII/CIBER-BBN info:eu-repo/semantics/openAccess by-nc-nd http://creativecommons.org/licenses/by-nc-nd/3.0/es/ 4.708 2018 PHARMACOLOGY & PHARMACY 28 / 266 = 0.105 2018 Q1 T1 1.326 2018 Biotechnology 2018 Q1 Pharmaceutical Science 2018 Q1 Medicine (miscellaneous) 2018 Q1 info:eu-repo/semantics/article info:eu-repo/semantics/acceptedVersion Skórska-Stania, A. Cierniak, A. (orcid)0000-0002-6873-5244 Sebastian, V. Universidad de Zaragoza Komarnicka, U.K. Plotek, M. Kyziol, A. 5005 555 Universidad de Zaragoza Dpto. Ing.Quím.Tecnol.Med.Amb. Área Ingeniería Química 128 (2018), 69-81 Eur. j. pharm. biopharm. European Journal of Pharmaceutics and Biopharmaceutics 0939-6411 2092332 http://zaguan.unizar.es/record/79071/files/texto_completo.pdf Postprint 53425 http://zaguan.unizar.es/record/79071/files/texto_completo.jpg?subformat=icon icon Postprint oai:zaguan.unizar.es:79071 articulos driver 2019-11-26-13:40:11 ARTICLE