000079078 001__ 79078
000079078 005__ 20191126134632.0
000079078 0247_ $$2doi$$a10.1016/j.cmi.2017.09.010
000079078 0248_ $$2sideral$$a106438
000079078 037__ $$aART-2018-106438
000079078 041__ $$aeng
000079078 100__ $$aOviaño, M.
000079078 245__ $$aDirect identification of clinical pathogens from liquid culture media by MALDI-TOF MS analysis
000079078 260__ $$c2018
000079078 5060_ $$aAccess copy available to the general public$$fUnrestricted
000079078 5203_ $$aObjectives: We propose using MALDI-TOF MS as a tool for identifying microorganisms directly from liquid cultures after enrichment of the clinical sample in the media, to obtain a rapid microbiological diagnosis and an adequate administration of the antibiotic therapy in a clinical setting.
Methods: To evaluate this approach, a series of quality control isolates were grown in thioglycollate (TG) broth and brain heart infusion (BHI) broth and extracted under four different protocols before finally being identified by MALDI-TOF MS. After establishing the best extraction protocol, we validated the method in a total of 300 liquid cultures (150 in TG broth and 150 in BHI broth) of different types of clinical samples obtained from two tertiary Spanish hospitals.
Results: The initial evaluation showed that the extraction protocol including a 5 minute sonication step yielded 100% valid identifications, with an average score value of 2.305. In the clinical validation of the procedure, 98% of the microorganisms identified from the TG broth were correctly identified relative to 97% of those identified from the BHI broth. In 24% of the samples analysed, growth by direct sowing was only successful in the liquid medium, and no growth was observed in the direct solid agar cultures.
Conclusions: Use of MALDI-TOF MS plus the sonication-based extraction method enabled direct and accurate identification of microorganisms in liquid culture media in 15 minutes, in contrast to the 24 hours of subculture required for conventional identification, allowing the administration of a targeted antimicrobial therapy.
000079078 536__ $$9info:eu-repo/grantAgreement/EUR/ERDF/A way to achieve Europe
000079078 540__ $$9info:eu-repo/semantics/openAccess$$aby-nc-nd$$uhttp://creativecommons.org/licenses/by-nc-nd/3.0/es/
000079078 590__ $$a6.425$$b2018
000079078 591__ $$aMICROBIOLOGY$$b17 / 133 = 0.128$$c2018$$dQ1$$eT1
000079078 591__ $$aINFECTIOUS DISEASES$$b6 / 89 = 0.067$$c2018$$dQ1$$eT1
000079078 592__ $$a2.651$$b2018
000079078 593__ $$aInfectious Diseases$$c2018$$dQ1
000079078 593__ $$aMicrobiology (medical)$$c2018$$dQ1
000079078 593__ $$aMedicine (miscellaneous)$$c2018$$dQ1
000079078 655_4 $$ainfo:eu-repo/semantics/article$$vinfo:eu-repo/semantics/acceptedVersion
000079078 700__ $$aRodríguez-Sánchez, B.
000079078 700__ $$aGómara, M.$$uUniversidad de Zaragoza
000079078 700__ $$aAlcalá, L.
000079078 700__ $$aZvezdanova, E.
000079078 700__ $$aRuíz, A.
000079078 700__ $$aVelasco, D.
000079078 700__ $$aGude, M.J.
000079078 700__ $$aBouza, E.
000079078 700__ $$aBou, G.
000079078 7102_ $$11008$$2630$$aUniversidad de Zaragoza$$bDpto. Microb.Med.Pr.,Sal.Públ.$$cÁrea Microbiología
000079078 773__ $$g24, 6 (2018), 624-629$$pClin. microbiol. infect.$$tClinical Microbiology and Infection$$x1198-743X
000079078 8564_ $$s361683$$uhttps://zaguan.unizar.es/record/79078/files/texto_completo.pdf$$yPostprint
000079078 8564_ $$s10898$$uhttps://zaguan.unizar.es/record/79078/files/texto_completo.jpg?subformat=icon$$xicon$$yPostprint
000079078 909CO $$ooai:zaguan.unizar.es:79078$$particulos$$pdriver
000079078 951__ $$a2019-11-26-13:41:34
000079078 980__ $$aARTICLE