000079103 001__ 79103
000079103 005__ 20240116083604.0
000079103 0247_ $$2doi$$a10.1016/j.actbio.2016.09.041
000079103 0248_ $$2sideral$$a97002
000079103 037__ $$aART-2017-97002
000079103 041__ $$aeng
000079103 100__ $$aCasciaro, Bruno
000079103 245__ $$aGold-nanoparticles coated with the antimicrobial peptide esculentin-1a(1-21)NH2 as a reliable strategy for antipseudomonal drugs
000079103 260__ $$c2017
000079103 5060_ $$aAccess copy available to the general public$$fUnrestricted
000079103 5203_ $$aNaturally occurring antimicrobial peptides (AMPs) hold promise as future therapeutics against multidrug resistant microorganisms. Recently, we have discovered that a derivative of the frog skin AMP esculentin-1a, Esc(1-21), is highly potent against both free living and biofilm forms of the bacterial pathogen Pseudomonas aeruginosa. However, bringing AMPs into clinics requires to overcome their low stability, high toxicity and inefficient delivery to the target site at high concentrations. Importantly, peptide conjugation to gold nanoparticles (AuNPs), which are among the most applied inorganic nanocarriers in biomedical sciences, represents a valuable strategy to solve these problems. Here we report that covalent conjugation of Esc(1-21) to soluble AuNPs AuNPs@Esc(1-21)] via a poly(ethylene glycol) linker increased by ~15-fold the activity of the free peptide against the motile and sessile forms of P. aeruginosa without being toxic to human keratinocytes. Furthermore, AuNPs@Esc(1-21) resulted to be significantly more resistant to proteolytic digestion and to disintegrate the bacterial membrane at very low concentration (5 nM). Finally, we demonstrated for the first time the capability of peptide-coated AuNPs to display a wound healing activity on a keratinocytes monolayer. Overall, these findings suggest that our engineered AuNPs can serve as attractive novel biological-derived material for topical treatment of epithelial infections and healing of the injured tissue. Statement of Significance Despite conjugation of AMPs to AuNPs represents a worthwhile solution to face some limitations for their development as new therapeutics, only a very limited number of studies is available on peptide-coated AuNPs. Importantly, this is the first report showing that a covalent binding of a linear AMP via a poly(ethylene glycol) linker to AuNPs highly enhances antipseudomonal activity, preserving the same mode of action of the free peptide, without being harmful. Furthermore, AuNPs@Esc(1-21) are expected to accelerate recovery of an injured skin layer. All together, these findings suggest our peptide-coated AuNPs as attractive novel nanoscale formulation to treat bacterial infections and to heal the injured tissue.
000079103 536__ $$9info:eu-repo/grantAgreement/ES/MINECO/SAF2014-54763-C2-2-R$$9info:eu-repo/grantAgreement/EC/FP7/239931/EU/Multifunctional Magnetic Nanoparticles: Towards Smart Drugs Design/NANOPUZZLE
000079103 540__ $$9info:eu-repo/semantics/openAccess$$aby-nc-nd$$uhttp://creativecommons.org/licenses/by-nc-nd/3.0/es/
000079103 590__ $$a6.383$$b2017
000079103 591__ $$aMATERIALS SCIENCE, BIOMATERIALS$$b3 / 33 = 0.091$$c2017$$dQ1$$eT1
000079103 591__ $$aENGINEERING, BIOMEDICAL$$b4 / 77 = 0.052$$c2017$$dQ1$$eT1
000079103 592__ $$a1.967$$b2017
000079103 593__ $$aBiochemistry$$c2017$$dQ1
000079103 593__ $$aBiomaterials$$c2017$$dQ1
000079103 593__ $$aBiomedical Engineering$$c2017$$dQ1
000079103 593__ $$aBiotechnology$$c2017$$dQ1
000079103 593__ $$aMedicine (miscellaneous)$$c2017$$dQ1
000079103 593__ $$aMolecular Biology$$c2017$$dQ2
000079103 655_4 $$ainfo:eu-repo/semantics/article$$vinfo:eu-repo/semantics/acceptedVersion
000079103 700__ $$0(orcid)0000-0002-2861-2469$$aMoros, Maria
000079103 700__ $$0(orcid)0000-0003-0616-4383$$aRivera-Fernández, Sara
000079103 700__ $$aBellelli, Andrea
000079103 700__ $$0(orcid)0000-0003-1081-8482$$aFuente, Jesús M. de la$$uUniversidad de Zaragoza
000079103 700__ $$aMangoni, María Luisa
000079103 7102_ $$12013$$2765$$aUniversidad de Zaragoza$$bDpto. Química Orgánica$$cÁrea Química Orgánica
000079103 773__ $$g47 (2017), 170-181$$pActa Biomater.$$tACTA BIOMATERIALIA$$x1742-7061
000079103 8564_ $$s5401027$$uhttps://zaguan.unizar.es/record/79103/files/texto_completo.pdf$$yPostprint
000079103 8564_ $$s50257$$uhttps://zaguan.unizar.es/record/79103/files/texto_completo.jpg?subformat=icon$$xicon$$yPostprint
000079103 909CO $$ooai:zaguan.unizar.es:79103$$particulos$$pdriver
000079103 951__ $$a2024-01-16-08:32:18
000079103 980__ $$aARTICLE