79139 20210526094557.0 doi 10.1016/j.trsl.2018.03.004 sideral 106599 ART-2018-106599 eng Alvarez-Arguedas, S. (orcid)0000-0002-5748-6078 Therapeutic efficacy of the live-attenuated Mycobacterium tuberculosis vaccine, MTBVAC, in a preclinical model of bladder cancer 2018 Access copy available to the general public Unrestricted Intravesical instillation of bacillus Calmette-Guérin (BCG) has been a first-line therapy for non–muscle-invasive bladder cancer for the last 4 decades. However, this treatment causes serious adverse events in a significant number of patients and a substantial percentage of recurrence episodes. MTBVAC is a live-attenuated vaccine derived from a Mycobacterium tuberculosis clinical isolate and is currently under evaluation in clinical trials to replace BCG as a tuberculosis vaccine. Here, we describe for the first time the potential of MTBVAC as a bladder cancer therapy in vitro and in vivo in a preclinical model. MTBVAC colonized human bladder tumor cells to a much greater extent than BCG via a mechanism mediated by macropinocytosis and induced cell growth inhibition after internalization. In vivo testing in an orthotopic murine model of bladder cancer demonstrated a higher antitumor effect of MTBVAC in experimental conditions in which BCG did not work. Our data encourage further studies to support the possible application of MTBVAC as a new immunotherapeutic agent for bladder cancer. info:eu-repo/grantAgreement/ES/DGA/FSE info:eu-repo/grantAgreement/ES/MINECO/BIO2014-5258P info:eu-repo/semantics/openAccess by-nc-nd http://creativecommons.org/licenses/by-nc-nd/3.0/es/ 4.915 2018 MEDICAL LABORATORY TECHNOLOGY 2 / 29 = 0.069 2018 Q1 T1 MEDICINE, RESEARCH & EXPERIMENTAL 22 / 135 = 0.163 2018 Q1 T1 MEDICINE, GENERAL & INTERNAL 19 / 159 = 0.119 2018 Q1 T1 1.803 2018 Biochemistry (medical) 2018 Q1 Public Health, Environmental and Occupational Health 2018 Q1 Physiology (medical) 2018 Q1 Medicine (miscellaneous) 2018 Q1 info:eu-repo/semantics/article info:eu-repo/semantics/acceptedVersion Uranga, S. Universidad de Zaragoza (orcid)0000-0001-7866-2803 Martín, M. Elizalde, J. Gomez, A.B. Universidad de Zaragoza (orcid)0000-0002-9713-2127 Julián, E. Nardelli-Haefliger, D. Martín, C. Universidad de Zaragoza (orcid)0000-0003-2993-5478 Aguilo, N. Universidad de Zaragoza (orcid)0000-0001-7897-9173 1008 630 Universidad de Zaragoza Dpto. Microb.Med.Pr.,Sal.Públ. Área Microbiología 1008 X Universidad de Zaragoza Dpto. Microb.Med.Pr.,Sal.Públ. Proy. investigación HQA 197 (2018), 32-42 Transl. Res. Translational Research 1931-5244 1051190 http://zaguan.unizar.es/record/79139/files/texto_completo.pdf Postprint 42716 http://zaguan.unizar.es/record/79139/files/texto_completo.jpg?subformat=icon icon Postprint oai:zaguan.unizar.es:79139 articulos driver 2021-05-26-09:31:33 ARTICLE