79317 20201029141734.0 doi 10.1111/jam.13343 sideral 97825 ART-2017-97825 eng (orcid)0000-0001-7559-1763 Andrés-Lasheras, S. Presence of Clostridium difficile in pig faecal samples and wild animal species associated with pig farms 2017 Access copy available to the general public Unrestricted Aims: to determine the presence of Clostridium difficile on fattening pig farms in north-eastern Spain. Methods and results: Twenty-seven farms were sampled. Pools of pig faecal samples (n = 210), samples of intestinal content from common farm pest species (n = 95) and environment-related samples (n = 93) were collected. Isolates were tested for toxin genes of C. difficile, and typed by PCR-ribotyping and toxinotyping. The minimal inhibitory concentrations of six antimicrobial agents were determined using Etest. Thirty-four isolates were obtained from 12 farms, and 30 (88·2%) had toxin genes. Seven ribotypes were identified. Ribotype 078 and its variant 126 were predominant (52·9%). The same ribotypes were isolated from different animal species on the same farm. None of the isolates were resistant to metronidazole or vancomycin. Conclusions: Clostridium difficile was common within the pig farm environment. Most of the positive samples came from pest species or were pest-related environmental samples. Significance and Impact of the Study: Pest species were colonized with toxigenic and antimicrobial-resistant C. difficile strains of the same ribotypes that are found in humans and pigs. Rodents and pigeons may transmit toxigenic and antimicrobial-resistant C. difficile strains that are of the same ribotypes as those occuring in humans. info:eu-repo/grantAgreement/ES/INIA/FAU2008-16 info:eu-repo/semantics/openAccess All rights reserved http://www.europeana.eu/rights/rr-f/ 2.16 2017 MICROBIOLOGY 79 / 125 = 0.632 2017 Q3 T2 BIOTECHNOLOGY & APPLIED MICROBIOLOGY 82 / 160 = 0.512 2017 Q3 T2 0.795 2017 Applied Microbiology and Biotechnology 2017 Q2 Medicine (miscellaneous) 2017 Q2 Biotechnology 2017 Q2 info:eu-repo/semantics/article info:eu-repo/semantics/acceptedVersion (orcid)0000-0002-2746-3932 Bolea, R. Universidad de Zaragoza (orcid)0000-0001-5442-7702 Mainar-Jaime, R. C. Universidad de Zaragoza Kuijper, E. (orcid)0000-0002-4213-2904 Sevilla, E. Universidad de Zaragoza (orcid)0000-0001-6016-4726 Martín-Burriel, I. Universidad de Zaragoza Chirino-Trejo, M. 1001 420 Universidad de Zaragoza Dpto. Anatom.,Embri.Genét.Ani. Área Genética 1009 773 Universidad de Zaragoza Dpto. Patología Animal Área Sanidad Animal 122, 2 (2017), 462-472 [27 p.] J. appl. microbiol. Journal of Applied Microbiology 1364-5072 285030 http://zaguan.unizar.es/record/79317/files/texto_completo.pdf Postprint 77009 http://zaguan.unizar.es/record/79317/files/texto_completo.jpg?subformat=icon icon Postprint oai:zaguan.unizar.es:79317 articulos driver 2020-10-29-14:05:18 ARTICLE