000079318 001__ 79318 000079318 005__ 20240105125717.0 000079318 0247_ $$2doi$$a10.1089/scd.2016.0209 000079318 0248_ $$2sideral$$a96455 000079318 037__ $$aART-2017-96455 000079318 041__ $$aeng 000079318 100__ $$0(orcid)0000-0001-9818-508X$$aBarrachina Porcar, Laura$$uUniversidad de Zaragoza 000079318 245__ $$aPriming equine bone marrow derived mesenchymal stem cells (eBM-MSCs) with pro-inflammatory cytokines: implications in immunomodulation-immunogenicity balance, cell viability and differentiation potential 000079318 260__ $$c2017 000079318 5060_ $$aAccess copy available to the general public$$fUnrestricted 000079318 5203_ $$aMesenchymal stem cells (MSCs) have a great potential for treating equine musculoskeletal injuries. Although their mechanisms of action are not completely known, their immunomodulatory properties appear to be key in their functions. The expression of immunoregulatory molecules by MSCs is regulated by proinflammatory cytokines; so inflammatory priming of MSCs might improve their therapeutic potential. However, inflammatory environment could also increase MSC immunogenicity and decrease MSC viability and differentiation capacity. The aim of this study was to assess the effect of cytokine priming on equine bone marrow-derived MSC (eBM-MSC) immunoregulation, immunogenicity, viability, and differentiation potential, to enhance MSC immunoregulatory properties, without impairing their immune-evasive status, viability, and plasticity. Equine BM-MSCs (n¿=¿4) were exposed to 5¿ng/mL of TNFa and IFN¿ for 12¿h (CK5-priming). Subsequently, expression of genes coding for immunomodulatory, immunogenic, and apoptosis-related molecules was analyzed by real-time quantitative polymerase chain reaction. Chromatin integrity and proliferation assays were assessed to evaluate cell viability. Trilineage differentiation was evaluated by specific staining and gene expression. Cells were reseeded in a basal medium for additional 7 days post-CK5 to elucidate if priming-induced changes were maintained along the time. CK5-priming led to an upregulation of immunoregulatory genes IDO, iNOS, IL-6, COX-2, and VCAM-1. MHC-II and CD40 were also upregulated, but no change in other costimulatory molecules was observed. These changes were not maintained 7 days after CK5-priming. Viability and differentiation potential were maintained after CK5-priming. These findings suggest that CK5-priming of eBM-MSCs could improve their in vivo effectiveness without affecting other eBM-MSC properties. 000079318 540__ $$9info:eu-repo/semantics/openAccess$$aAll rights reserved$$uhttp://www.europeana.eu/rights/rr-f/ 000079318 590__ $$a3.315$$b2017 000079318 591__ $$aHEMATOLOGY$$b24 / 71 = 0.338$$c2017$$dQ2$$eT2 000079318 591__ $$aTRANSPLANTATION$$b9 / 25 = 0.36$$c2017$$dQ2$$eT2 000079318 591__ $$aMEDICINE, RESEARCH & EXPERIMENTAL$$b51 / 133 = 0.383$$c2017$$dQ2$$eT2 000079318 591__ $$aCELL & TISSUE ENGINEERING$$b14 / 24 = 0.583$$c2017$$dQ3$$eT2 000079318 592__ $$a1.426$$b2017 000079318 593__ $$aHematology$$c2017$$dQ1 000079318 593__ $$aCell Biology$$c2017$$dQ2 000079318 593__ $$aDevelopmental Biology$$c2017$$dQ2 000079318 655_4 $$ainfo:eu-repo/semantics/article$$vinfo:eu-repo/semantics/acceptedVersion 000079318 700__ $$0(orcid)0000-0002-1075-8267$$aRemacha Gayán, Ana Rosa$$uUniversidad de Zaragoza 000079318 700__ $$0(orcid)0000-0001-7188-0461$$aRomero Lasheras, Antonio$$uUniversidad de Zaragoza 000079318 700__ $$0(orcid)0000-0002-8712-2275$$aVázquez Bringas, Francisco José$$uUniversidad de Zaragoza 000079318 700__ $$0(orcid)0000-0003-4489-3130$$aAlbareda, Jorge$$uUniversidad de Zaragoza 000079318 700__ $$0(orcid)0000-0003-2713-9232$$aPrades, Marta 000079318 700__ $$aGosálvez, Jaime 000079318 700__ $$aRoy, Rosa 000079318 700__ $$0(orcid)0000-0001-5740-0185$$aZaragoza, Pilar$$uUniversidad de Zaragoza 000079318 700__ $$0(orcid)0000-0001-6016-4726$$aMartín Burriel, Inmaculada$$uUniversidad de Zaragoza 000079318 700__ $$0(orcid)0000-0003-3289-2675$$aRodellar Penella, Clementina$$uUniversidad de Zaragoza 000079318 7102_ $$11001$$2420$$aUniversidad de Zaragoza$$bDpto. Anatom.,Embri.Genét.Ani.$$cÁrea Genética 000079318 7102_ $$11004$$2830$$aUniversidad de Zaragoza$$bDpto. Cirugía,Ginecol.Obstetr.$$cÁrea Traumatología y Ortopedia 000079318 7102_ $$11009$$2617$$aUniversidad de Zaragoza$$bDpto. Patología Animal$$cÁrea Medicina y Cirugía Animal 000079318 773__ $$g26, 1 (2017), 15-24$$pStem Cells Dev.$$tSTEM CELLS AND DEVELOPMENT$$x1547-3287 000079318 8564_ $$s199018$$uhttps://zaguan.unizar.es/record/79318/files/texto_completo.pdf$$yPostprint 000079318 8564_ $$s77808$$uhttps://zaguan.unizar.es/record/79318/files/texto_completo.jpg?subformat=icon$$xicon$$yPostprint 000079318 909CO $$ooai:zaguan.unizar.es:79318$$particulos$$pdriver 000079318 951__ $$a2024-01-05-12:46:26 000079318 980__ $$aARTICLE