79318 20240105125717.0 doi 10.1089/scd.2016.0209 sideral 96455 ART-2017-96455 eng Barrachina Porcar, Laura Universidad de Zaragoza (orcid)0000-0001-9818-508X Priming equine bone marrow derived mesenchymal stem cells (eBM-MSCs) with pro-inflammatory cytokines: implications in immunomodulation-immunogenicity balance, cell viability and differentiation potential 2017 Access copy available to the general public Unrestricted Mesenchymal stem cells (MSCs) have a great potential for treating equine musculoskeletal injuries. Although their mechanisms of action are not completely known, their immunomodulatory properties appear to be key in their functions. The expression of immunoregulatory molecules by MSCs is regulated by proinflammatory cytokines; so inflammatory priming of MSCs might improve their therapeutic potential. However, inflammatory environment could also increase MSC immunogenicity and decrease MSC viability and differentiation capacity. The aim of this study was to assess the effect of cytokine priming on equine bone marrow-derived MSC (eBM-MSC) immunoregulation, immunogenicity, viability, and differentiation potential, to enhance MSC immunoregulatory properties, without impairing their immune-evasive status, viability, and plasticity. Equine BM-MSCs (n¿=¿4) were exposed to 5¿ng/mL of TNFa and IFN¿ for 12¿h (CK5-priming). Subsequently, expression of genes coding for immunomodulatory, immunogenic, and apoptosis-related molecules was analyzed by real-time quantitative polymerase chain reaction. Chromatin integrity and proliferation assays were assessed to evaluate cell viability. Trilineage differentiation was evaluated by specific staining and gene expression. Cells were reseeded in a basal medium for additional 7 days post-CK5 to elucidate if priming-induced changes were maintained along the time. CK5-priming led to an upregulation of immunoregulatory genes IDO, iNOS, IL-6, COX-2, and VCAM-1. MHC-II and CD40 were also upregulated, but no change in other costimulatory molecules was observed. These changes were not maintained 7 days after CK5-priming. Viability and differentiation potential were maintained after CK5-priming. These findings suggest that CK5-priming of eBM-MSCs could improve their in vivo effectiveness without affecting other eBM-MSC properties. info:eu-repo/semantics/openAccess All rights reserved http://www.europeana.eu/rights/rr-f/ 3.315 2017 HEMATOLOGY 24 / 71 = 0.338 2017 Q2 T2 TRANSPLANTATION 9 / 25 = 0.36 2017 Q2 T2 MEDICINE, RESEARCH & EXPERIMENTAL 51 / 133 = 0.383 2017 Q2 T2 CELL & TISSUE ENGINEERING 14 / 24 = 0.583 2017 Q3 T2 1.426 2017 Hematology 2017 Q1 Cell Biology 2017 Q2 Developmental Biology 2017 Q2 info:eu-repo/semantics/article info:eu-repo/semantics/acceptedVersion Remacha Gayán, Ana Rosa Universidad de Zaragoza (orcid)0000-0002-1075-8267 Romero Lasheras, Antonio Universidad de Zaragoza (orcid)0000-0001-7188-0461 Vázquez Bringas, Francisco José Universidad de Zaragoza (orcid)0000-0002-8712-2275 Albareda, Jorge Universidad de Zaragoza (orcid)0000-0003-4489-3130 Prades, Marta (orcid)0000-0003-2713-9232 Gosálvez, Jaime Roy, Rosa Zaragoza, Pilar Universidad de Zaragoza (orcid)0000-0001-5740-0185 Martín Burriel, Inmaculada Universidad de Zaragoza (orcid)0000-0001-6016-4726 Rodellar Penella, Clementina Universidad de Zaragoza (orcid)0000-0003-3289-2675 1001 420 Universidad de Zaragoza Dpto. Anatom.,Embri.Genét.Ani. Área Genética 1004 830 Universidad de Zaragoza Dpto. Cirugía,Ginecol.Obstetr. Área Traumatología y Ortopedia 1009 617 Universidad de Zaragoza Dpto. Patología Animal Área Medicina y Cirugía Animal 26, 1 (2017), 15-24 Stem Cells Dev. STEM CELLS AND DEVELOPMENT 1547-3287 199018 http://zaguan.unizar.es/record/79318/files/texto_completo.pdf Postprint 77808 http://zaguan.unizar.es/record/79318/files/texto_completo.jpg?subformat=icon icon Postprint oai:zaguan.unizar.es:79318 articulos driver 2024-01-05-12:46:26 ARTICLE