000079344 001__ 79344
000079344 005__ 20191127155458.0
000079344 0247_ $$2doi$$a10.1177/0885328217739199
000079344 0248_ $$2sideral$$a103752
000079344 037__ $$aART-2018-103752
000079344 041__ $$aeng
000079344 100__ $$0(orcid)0000-0003-2293-363X$$aMendoza, Gracia
000079344 245__ $$aChitosan-based coatings in the prevention of intravascular catheter-associated infections
000079344 260__ $$c2018
000079344 5060_ $$aAccess copy available to the general public$$fUnrestricted
000079344 5203_ $$aCentral venous access devices play an important role in patients with prolonged intravenous administration requirements. In the last years, the coating of these devices with bactericidal compounds has emerged as a potential tool to prevent bacterial colonization. Our study describes the modification of 3D-printed reservoirs and silicone-based catheters, mimicking central venous access devices, through different approaches including their coating with the well known biocompatible and bactericidal polymer chitosan, with the anionic polysaccharide alginate; also, plasma treated surfaces were included in the study to promote polymer adhesion. The evaluation of the antimicrobial action of those surface modifications compared to that exerted by a model antibiotic (ciprofloxacin) adsorbed on the surface of the devices was carried out. Surface characterization was developed by different methodologies and the bactericidal effects of the different coatings were assayed in an in vitro model of Staphylococcus aureus infection. Our results showed a significant reduction in the reservoir roughness (=73%) after coating though no changes were observed for coated catheters which was also confirmed by scanning electron microscopy, pointing to the importance of the surface device topography for the successful attachment of the coating and for the subsequent development of bactericidal effects. Furthermore, the single presence of chitosan on the reservoirs was enough to fully inhibit bacterial growth exerting the same efficiency as that showed by the model antibiotic. Importantly, chitosan coating showed low cytotoxicity against human keratinocytes, human lung adenocarcinoma epithelial cells, and murine colon carcinoma cells displaying viability percentages in the range of the control samples (>95%). Chitosan-based coatings are proposed as an effective and promising solution in the prevention of microbial infections associated to medical devices.
000079344 536__ $$9info:eu-repo/grantAgreement/EC/FP7/614715/EU/A Photo-triggered On-demand Drug Delivery System for Chronic Pain/NANOHEDONISM$$9info:eu-repo/grantAgreement/ES/MINECO/Consolider-Ingenio2010
000079344 540__ $$9info:eu-repo/semantics/openAccess$$aAll rights reserved$$uhttp://www.europeana.eu/rights/rr-f/
000079344 590__ $$a2.442$$b2018
000079344 591__ $$aENGINEERING, BIOMEDICAL$$b35 / 80 = 0.438$$c2018$$dQ2$$eT2
000079344 591__ $$aMATERIALS SCIENCE, BIOMATERIALS$$b22 / 32 = 0.688$$c2018$$dQ3$$eT3
000079344 592__ $$a0.572$$b2018
000079344 593__ $$aBiomedical Engineering$$c2018$$dQ2
000079344 593__ $$aBiomaterials$$c2018$$dQ2
000079344 655_4 $$ainfo:eu-repo/semantics/article$$vinfo:eu-repo/semantics/acceptedVersion
000079344 700__ $$aRegiel-Gutyra, Anna
000079344 700__ $$aTamayo, Alejandra
000079344 700__ $$0(orcid)0000-0002-2787-9671$$aMonzon, Marta$$uUniversidad de Zaragoza
000079344 700__ $$0(orcid)0000-0002-2966-9088$$aIrusta, Silvia$$uUniversidad de Zaragoza
000079344 700__ $$0(orcid)0000-0002-5618-7519$$aGregorio, Miguel Ángel de$$uUniversidad de Zaragoza
000079344 700__ $$aKyziol, Agnieszka
000079344 700__ $$0(orcid)0000-0003-3165-0156$$aArruebo, Manuel$$uUniversidad de Zaragoza
000079344 7102_ $$11010$$2770$$aUniversidad de Zaragoza$$bDpto. Pediatría Radiol.Med.Fís$$cÁrea Radiol. y Medicina Física
000079344 7102_ $$11003$$2443$$aUniversidad de Zaragoza$$bDpto. Anatom.Histolog.Humanas$$cArea Histología
000079344 7102_ $$15005$$2555$$aUniversidad de Zaragoza$$bDpto. Ing.Quím.Tecnol.Med.Amb.$$cÁrea Ingeniería Química
000079344 773__ $$g32, 6 (2018), 725-737$$pJ. biomater. appl.$$tJOURNAL OF BIOMATERIALS APPLICATIONS$$x0885-3282
000079344 8564_ $$s1253390$$uhttps://zaguan.unizar.es/record/79344/files/texto_completo.pdf$$yPostprint
000079344 8564_ $$s53624$$uhttps://zaguan.unizar.es/record/79344/files/texto_completo.jpg?subformat=icon$$xicon$$yPostprint
000079344 909CO $$ooai:zaguan.unizar.es:79344$$particulos$$pdriver
000079344 951__ $$a2019-11-27-15:48:40
000079344 980__ $$aARTICLE