79344 20191127155458.0 doi 10.1177/0885328217739199 sideral 103752 ART-2018-103752 eng (orcid)0000-0003-2293-363X Mendoza, Gracia Chitosan-based coatings in the prevention of intravascular catheter-associated infections 2018 Access copy available to the general public Unrestricted Central venous access devices play an important role in patients with prolonged intravenous administration requirements. In the last years, the coating of these devices with bactericidal compounds has emerged as a potential tool to prevent bacterial colonization. Our study describes the modification of 3D-printed reservoirs and silicone-based catheters, mimicking central venous access devices, through different approaches including their coating with the well known biocompatible and bactericidal polymer chitosan, with the anionic polysaccharide alginate; also, plasma treated surfaces were included in the study to promote polymer adhesion. The evaluation of the antimicrobial action of those surface modifications compared to that exerted by a model antibiotic (ciprofloxacin) adsorbed on the surface of the devices was carried out. Surface characterization was developed by different methodologies and the bactericidal effects of the different coatings were assayed in an in vitro model of Staphylococcus aureus infection. Our results showed a significant reduction in the reservoir roughness (=73%) after coating though no changes were observed for coated catheters which was also confirmed by scanning electron microscopy, pointing to the importance of the surface device topography for the successful attachment of the coating and for the subsequent development of bactericidal effects. Furthermore, the single presence of chitosan on the reservoirs was enough to fully inhibit bacterial growth exerting the same efficiency as that showed by the model antibiotic. Importantly, chitosan coating showed low cytotoxicity against human keratinocytes, human lung adenocarcinoma epithelial cells, and murine colon carcinoma cells displaying viability percentages in the range of the control samples (>95%). Chitosan-based coatings are proposed as an effective and promising solution in the prevention of microbial infections associated to medical devices. info:eu-repo/grantAgreement/EC/FP7/614715/EU/A Photo-triggered On-demand Drug Delivery System for Chronic Pain/NANOHEDONISM info:eu-repo/grantAgreement/ES/MINECO/Consolider-Ingenio2010 info:eu-repo/semantics/openAccess All rights reserved http://www.europeana.eu/rights/rr-f/ 2.442 2018 ENGINEERING, BIOMEDICAL 35 / 80 = 0.438 2018 Q2 T2 MATERIALS SCIENCE, BIOMATERIALS 22 / 32 = 0.688 2018 Q3 T3 0.572 2018 Biomedical Engineering 2018 Q2 Biomaterials 2018 Q2 info:eu-repo/semantics/article info:eu-repo/semantics/acceptedVersion Regiel-Gutyra, Anna Tamayo, Alejandra (orcid)0000-0002-2787-9671 Monzon, Marta Universidad de Zaragoza (orcid)0000-0002-2966-9088 Irusta, Silvia Universidad de Zaragoza (orcid)0000-0002-5618-7519 Gregorio, Miguel Ángel de Universidad de Zaragoza Kyziol, Agnieszka (orcid)0000-0003-3165-0156 Arruebo, Manuel Universidad de Zaragoza 1010 770 Universidad de Zaragoza Dpto. Pediatría Radiol.Med.Fís Área Radiol. y Medicina Física 1003 443 Universidad de Zaragoza Dpto. Anatom.Histolog.Humanas Area Histología 5005 555 Universidad de Zaragoza Dpto. Ing.Quím.Tecnol.Med.Amb. Área Ingeniería Química 32, 6 (2018), 725-737 J. biomater. appl. JOURNAL OF BIOMATERIALS APPLICATIONS 0885-3282 1253390 http://zaguan.unizar.es/record/79344/files/texto_completo.pdf Postprint 53624 http://zaguan.unizar.es/record/79344/files/texto_completo.jpg?subformat=icon icon Postprint oai:zaguan.unizar.es:79344 articulos driver 2019-11-27-15:48:40 ARTICLE