000079552 001__ 79552 000079552 005__ 20230126102835.0 000079552 0247_ $$2doi$$a10.1016/j.foodcont.2018.06.031 000079552 0248_ $$2sideral$$a106818 000079552 037__ $$aART-2018-106818 000079552 041__ $$aeng 000079552 100__ $$0(orcid)0000-0001-9123-4037$$aLabrador, M.$$uUniversidad de Zaragoza 000079552 245__ $$aComparative evaluation of impedanciometry combined with chromogenic agars or RNA hybridization and real-time PCR methods for the detection of L. monocytogenes in dry-cured ham 000079552 260__ $$c2018 000079552 5060_ $$aAccess copy available to the general public$$fUnrestricted 000079552 5203_ $$aListeria monocytogenes is an important foodborne pathogen of particular relevance in “Ready To Eat” products. Food producers require rapid methods to detect L. monocytogenes, since the reference method (ISO 11290-1) is laborious, lengthy and costly. The aim of this study was to evaluate three alternative methods to detect L. monocytogenes in dry-cured ham following the ISO 16140-2:2016 standard: (A) impedance measurement followed by plating onto chromogenic agars; (B) impedance measurement followed by RNA hybridization, and (C) real-time PCR. Inclusivity and exclusivity were evaluated. The limits of detection 50 (LOD50) and the relative limits of detection (RLOD) were obtained by analysing dry-cured ham samples inoculated with L. monocytogenes at three different levels of contamination. The sensitivity study of alternative methods, as well as the relative specificity (SP), sensitivity (SE), and Kappa Cohen''s index were calculated analysing 93 samples of sliced dry-cured ham. The inclusivity and exclusivity tests of three methods showed no interference in pathogen detection. LOD50 were very low for the three methods evaluated (<1 cfu/25 g dry-cured ham). The RLOD values of the three alternative methods were below the acceptability limit established by ISO 16140. For methods A and C, good results were obtained in the sensitivity study, as well as in the SP and SE. However, method B showed poorer results in the sensitivity study, along with lower results for SP (99.7%) and SE (79.6%), due to the occurrence of false positives and negatives in samples with presence of other Listeria spp. Methods A and C were considered to be a thoroughly appropriate control tool for use in the meat industry to improve the detection of L. monocytogenes. 000079552 536__ $$9info:eu-repo/grantAgreement/ES/DGA-FEDER/A06-17R$$9info:eu-repo/grantAgreement/ES/DGA-FSE/C091-2014$$9info:eu-repo/grantAgreement/ES/MINECO-FEDER/INNPACTO-IPT-2012-0189-060000 000079552 540__ $$9info:eu-repo/semantics/openAccess$$aAll rights reserved$$uhttp://www.europeana.eu/rights/rr-f/ 000079552 590__ $$a4.248$$b2018 000079552 591__ $$aFOOD SCIENCE & TECHNOLOGY$$b10 / 135 = 0.074$$c2018$$dQ1$$eT1 000079552 592__ $$a1.45$$b2018 000079552 593__ $$aFood Science$$c2018$$dQ1 000079552 593__ $$aBiotechnology$$c2018$$dQ1 000079552 655_4 $$ainfo:eu-repo/semantics/article$$vinfo:eu-repo/semantics/acceptedVersion 000079552 700__ $$0(orcid)0000-0001-6375-9784$$aRota, M.C.$$uUniversidad de Zaragoza 000079552 700__ $$0(orcid)0000-0003-2247-0954$$aPérez-Arquillué, C.$$uUniversidad de Zaragoza 000079552 700__ $$0(orcid)0000-0001-7195-3640$$aHerrera, A.$$uUniversidad de Zaragoza 000079552 700__ $$0(orcid)0000-0002-1668-4940$$aBayarri, S.$$uUniversidad de Zaragoza 000079552 7102_ $$12008$$2640$$aUniversidad de Zaragoza$$bDpto. Produc.Animal Cienc.Ali.$$cÁrea Nutrición Bromatología 000079552 773__ $$g94 (2018), 108-115$$pFood control$$tFood Control$$x0956-7135 000079552 8564_ $$s209045$$uhttps://zaguan.unizar.es/record/79552/files/texto_completo.pdf$$yPostprint 000079552 8564_ $$s7925$$uhttps://zaguan.unizar.es/record/79552/files/texto_completo.jpg?subformat=icon$$xicon$$yPostprint 000079552 909CO $$ooai:zaguan.unizar.es:79552$$particulos$$pdriver 000079552 951__ $$a2023-01-26-09:52:52 000079552 980__ $$aARTICLE