79629 20200716101445.0 doi 10.3390/ma12071092 sideral 112231 ART-2019-112231 eng Comas, Laura Intracellular delivery of biologically-active fungal metabolite gliotoxin using magnetic nanoparticles 2019 Access copy available to the general public Unrestricted Gliotoxin (GT), a secondary metabolite produced by Aspergillus molds, has been proposed as a potential anti-tumor agent. Here we have developed a nanoparticle approach to enhance delivery of GT in tumor cells and establish a basis for its potential use as therapeutical drug. GT bound to magnetic nanoparticles (MNPs) retained a high anti-tumor activity, correlating with efficient intracellular delivery, which was increased in the presence of glucose. Our results show that the attachment of GT to MNPs by covalent bonding enhances intracellular GT delivery without affecting its biological activity. This finding represents the first step to use this potent anti-tumor agent in the treatment of cancer. info:eu-repo/grantAgreement/ES/MINECO/FJCI-2017-31629 info:eu-repo/grantAgreement/ES/MINECO/SAF2014-54763-C2-1-R info:eu-repo/grantAgreement/ES/MINECO/SAF2014-54763-C2-2-R info:eu-repo/grantAgreement/ES/MINECO/SAF2017-83120-C2-1-R info:eu-repo/semantics/openAccess by http://creativecommons.org/licenses/by/3.0/es/ 3.057 2019 MATERIALS SCIENCE, MULTIDISCIPLINARY 132 / 314 = 0.42 2019 Q2 T2 0.647 2019 Materials Science (miscellaneous) 2019 Q2 Condensed Matter Physics 2019 Q2 info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion Polo, Esther Domingo, M. Pilar Hernandez, Yulán (orcid)0000-0002-9730-2210 Arias, Maykel Esteban, Patricia (orcid)0000-0003-3043-147X Martinez-Lostao, Luis Universidad de Zaragoza (orcid)0000-0003-0154-0730 Pardo, Julián Universidad de Zaragoza (orcid)0000-0003-1081-8482 Martínez de la Fuente, Jesús (orcid)0000-0001-6928-5516 Galvez, Eva M. 1008 566 Universidad de Zaragoza Dpto. Microb.Med.Pr.,Sal.Públ. Área Inmunología 12, 7 (2019), 1092 [17 pp] MATERIALS Materials 1996-1944 4095993 http://zaguan.unizar.es/record/79629/files/texto_completo.pdf Versión publicada 102363 http://zaguan.unizar.es/record/79629/files/texto_completo.jpg?subformat=icon icon Versión publicada oai:zaguan.unizar.es:79629 articulos driver 2020-07-16-09:02:12 ARTICLE