000079689 001__ 79689
000079689 005__ 20240105103346.0
000079689 0247_ $$2doi$$a10.1186/s13071-019-3467-y
000079689 0248_ $$2sideral$$a112463
000079689 037__ $$aART-2019-112463
000079689 041__ $$aeng
000079689 100__ $$aHernández-Triana, Luis M.
000079689 245__ $$aEvidence for infection but not transmission of Zika virus by Aedes albopictus (Diptera: Culicidae) from Spain
000079689 260__ $$c2019
000079689 5060_ $$aAccess copy available to the general public$$fUnrestricted
000079689 5203_ $$aBackground: A number of mosquito-borne viruses such as dengue virus (DENV), Usutu virus (USUV), West Nile virus (WNV) are autochthonously transmitted in Europe and six invasive mosquito species have been detected in this temperate region. This has increased the risk for the emergence of further mosquito-borne diseases. However, there is a paucity of information on whether European populations of invasive mosquito species are competent to transmit arboviruses. In this study, the susceptibility of Aedes albopictus originating from Spain and a laboratory-adapted colony of Aedes aegypti, was assessed for infection with, and transmission of Zika virus (ZIKV). Vertical transmission in both species was also assessed. Methods: Aedes albopictus colonised from eggs collected in Spain and an existing colony of Ae. aegypti were fed infectious blood meals containing ZIKV (Polynesian strain) at 1.6 × 10 7 PFU/ml. Blood-fed mosquitoes were separated and maintained at 20 °C or 25 °C. Legs, saliva and bodies were sampled from specimens at 7, 14 and 21 days post-infection (dpi) in order to determine infection, dissemination and transmission rates. All samples were analysed by real-time RT-PCR using primers targeting the ZIKV NS1 gene. Results: At 14 dpi and 21 dpi, ZIKV RNA was detected in the bodies of both species at both temperatures. However, live virus only was detected in the saliva of Ae. aegypti at 25 °C with a transmission rate of 44%. No evidence for virus expectoration was obtained for Ae. albopictus under any condition. Notably, ZIKV RNA was not detectable in the saliva of Ae. aegypti at 20 °C after 21 days. No vertical transmission of ZIKV was detected in this study. Conclusions: Experimental infection of Ae. albopictus colonized from Spain with ZIKV did not result in expectoration of virus in saliva in contrast to results for Ae. aegypti. No evidence of vertical transmission of virus was observed in this study. This suggests that this strain of Ae. albopictus is not competent for ZIKV transmission under the conditions tested. © 2019 The Author(s).
000079689 536__ $$9info:eu-repo/grantAgreement/EC/H2020/653316/EU/European Virus Archive goes global/EVAg$$9This project has received funding from the European Union’s Horizon 2020 research and innovation program under grant agreement No H2020 653316-EVAg
000079689 540__ $$9info:eu-repo/semantics/openAccess$$aby$$uhttp://creativecommons.org/licenses/by/3.0/es/
000079689 590__ $$a2.824$$b2019
000079689 592__ $$a1.406$$b2019
000079689 591__ $$aTROPICAL MEDICINE$$b3 / 23 = 0.13$$c2019$$dQ1$$eT1
000079689 593__ $$aParasitology$$c2019$$dQ1
000079689 591__ $$aPARASITOLOGY$$b9 / 39 = 0.231$$c2019$$dQ1$$eT1
000079689 593__ $$aInfectious Diseases$$c2019$$dQ1
000079689 655_4 $$ainfo:eu-repo/semantics/article$$vinfo:eu-repo/semantics/publishedVersion
000079689 700__ $$aBarrero, Elsa
000079689 700__ $$0(orcid)0000-0001-7046-2997$$aDelacour-Estrella, Sarah$$uUniversidad de Zaragoza
000079689 700__ $$0(orcid)0000-0001-8198-8118$$aRuiz-Arrondo, Ignacio$$uUniversidad de Zaragoza
000079689 700__ $$0(orcid)0000-0003-0663-8411$$aLucientes, Javier$$uUniversidad de Zaragoza
000079689 700__ $$aFernández de Marco, María del Mar
000079689 700__ $$aThorne, Leigh
000079689 700__ $$aLumley, Sarah
000079689 700__ $$aJohnson, Nicholas
000079689 700__ $$aMansfield, Karen L.
000079689 700__ $$aFooks, Anthony R.
000079689 7102_ $$11009$$2773$$aUniversidad de Zaragoza$$bDpto. Patología Animal$$cÁrea Sanidad Animal
000079689 773__ $$g12 (2019), 204 [6 pp.]$$pParasites & Vectors$$tParasites and Vectors$$x1756-3305
000079689 8564_ $$s782685$$uhttps://zaguan.unizar.es/record/79689/files/texto_completo.pdf$$yVersión publicada
000079689 8564_ $$s8227$$uhttps://zaguan.unizar.es/record/79689/files/texto_completo.jpg?subformat=icon$$xicon$$yVersión publicada
000079689 909CO $$ooai:zaguan.unizar.es:79689$$particulos$$pdriver
000079689 951__ $$a2024-01-05-10:13:47
000079689 980__ $$aARTICLE