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000079718 005__ 20200117221644.0
000079718 0247_ $$2doi$$a10.1002/bmb.21136
000079718 0248_ $$2sideral$$a107909
000079718 037__ $$aART-2018-107909
000079718 041__ $$aeng
000079718 100__ $$0(orcid)0000-0002-0531-0943$$aGonzález Rodríguez, Andrés$$uUniversidad de Zaragoza
000079718 245__ $$aOverexpression, immunodetection and site-directed mutagenesis of Anabaena sp. PCC 7120 flavodoxin: a comprehensive laboratory practice on molecular biology
000079718 260__ $$c2018
000079718 5060_ $$aAccess copy available to the general public$$fUnrestricted
000079718 5203_ $$aRecombinant protein expression and site-directed mutagenesis of target genes have demonstrated an increasing importance in the fields of molecular biology, biochemistry, biotechnology, and medicine. By using the flavodoxin of the model cyanobacterium Anabaena sp. PCC 7120 as a laboratory tool, we designed a comprehensive laboratory practice encompassing several well-established molecular biology techniques and procedures in order to fulfill two main objectives: (1) overexpression and immunodetection of Anabaena flavodoxin in recombinant Escherichia coli cell extracts, and (2) site-directed mutagenesis of the Anabaena flavodoxin gene isiB. This lab practice provides undergraduate students the possibility to perform by themselves several essential techniques in the field. With the aid of professors, students are stimulated to think, to interpret, and to discuss the results based on what they had learned in previous theoretical courses.
000079718 540__ $$9info:eu-repo/semantics/openAccess$$aAll rights reserved$$uhttp://www.europeana.eu/rights/rr-f/
000079718 590__ $$a0.947$$b2018
000079718 591__ $$aEDUCATION, SCIENTIFIC DISCIPLINES$$b32 / 41 = 0.78$$c2018$$dQ4$$eT3
000079718 591__ $$aBIOCHEMISTRY & MOLECULAR BIOLOGY$$b277 / 294 = 0.942$$c2018$$dQ4$$eT3
000079718 592__ $$a0.392$$b2018
000079718 593__ $$aMolecular Biology$$c2018$$dQ3
000079718 593__ $$aBiochemistry$$c2018$$dQ3
000079718 655_4 $$ainfo:eu-repo/semantics/article$$vinfo:eu-repo/semantics/acceptedVersion
000079718 700__ $$0(orcid)0000-0001-8644-4574$$aFillat, María F.$$uUniversidad de Zaragoza
000079718 7102_ $$11002$$2060$$aUniversidad de Zaragoza$$bDpto. Bioq.Biolog.Mol. Celular$$cÁrea Bioquímica y Biolog.Mole.
000079718 773__ $$g46, 5 (2018), 493-501$$pBiochem. Mol. Biol. Educ$$tBIOCHEMISTRY AND MOLECULAR BIOLOGY EDUCATION$$x1470-8175
000079718 8564_ $$s767248$$uhttps://zaguan.unizar.es/record/79718/files/texto_completo.pdf$$yPostprint
000079718 8564_ $$s112292$$uhttps://zaguan.unizar.es/record/79718/files/texto_completo.jpg?subformat=icon$$xicon$$yPostprint
000079718 909CO $$ooai:zaguan.unizar.es:79718$$particulos$$pdriver
000079718 951__ $$a2020-01-17-22:05:34
000079718 980__ $$aARTICLE