79718 20200117221644.0 doi 10.1002/bmb.21136 sideral 107909 ART-2018-107909 eng (orcid)0000-0002-0531-0943 González Rodríguez, Andrés Universidad de Zaragoza Overexpression, immunodetection and site-directed mutagenesis of Anabaena sp. PCC 7120 flavodoxin: a comprehensive laboratory practice on molecular biology 2018 Access copy available to the general public Unrestricted Recombinant protein expression and site-directed mutagenesis of target genes have demonstrated an increasing importance in the fields of molecular biology, biochemistry, biotechnology, and medicine. By using the flavodoxin of the model cyanobacterium Anabaena sp. PCC 7120 as a laboratory tool, we designed a comprehensive laboratory practice encompassing several well-established molecular biology techniques and procedures in order to fulfill two main objectives: (1) overexpression and immunodetection of Anabaena flavodoxin in recombinant Escherichia coli cell extracts, and (2) site-directed mutagenesis of the Anabaena flavodoxin gene isiB. This lab practice provides undergraduate students the possibility to perform by themselves several essential techniques in the field. With the aid of professors, students are stimulated to think, to interpret, and to discuss the results based on what they had learned in previous theoretical courses. info:eu-repo/semantics/openAccess All rights reserved http://www.europeana.eu/rights/rr-f/ 0.947 2018 EDUCATION, SCIENTIFIC DISCIPLINES 32 / 41 = 0.78 2018 Q4 T3 BIOCHEMISTRY & MOLECULAR BIOLOGY 277 / 294 = 0.942 2018 Q4 T3 0.392 2018 Molecular Biology 2018 Q3 Biochemistry 2018 Q3 info:eu-repo/semantics/article info:eu-repo/semantics/acceptedVersion (orcid)0000-0001-8644-4574 Fillat, María F. Universidad de Zaragoza 1002 060 Universidad de Zaragoza Dpto. Bioq.Biolog.Mol. Celular Área Bioquímica y Biolog.Mole. 46, 5 (2018), 493-501 Biochem. Mol. Biol. Educ BIOCHEMISTRY AND MOLECULAR BIOLOGY EDUCATION 1470-8175 767248 http://zaguan.unizar.es/record/79718/files/texto_completo.pdf Postprint 112292 http://zaguan.unizar.es/record/79718/files/texto_completo.jpg?subformat=icon icon Postprint oai:zaguan.unizar.es:79718 articulos driver 2020-01-17-22:05:34 ARTICLE