000079752 001__ 79752 000079752 005__ 20200716101455.0 000079752 0247_ $$2doi$$a10.3390/en12132614 000079752 0248_ $$2sideral$$a112602 000079752 037__ $$aART-2019-112602 000079752 041__ $$aeng 000079752 100__ $$aPortero Barahona, Patricia 000079752 245__ $$aAssessment of the effect of nitrogen concentration on fermentation and selection of a highly competitive Saccharomyces cerevisiae strain for efficient ethanol production 000079752 260__ $$c2019 000079752 5060_ $$aAccess copy available to the general public$$fUnrestricted 000079752 5203_ $$aThe optimum nitrogen concentration for media supplementation and strain dominance are aspects of key importance to the industrial production of ethanol with a view to reducing costs and increasing yields. In this work, these two factors were investigated for four ethanologenic Saccharomyces cerevisiae strains (CLQCA-INT-001, CLQCA-INT-005, CLQCA-10-099, and UCLM 325), selected from the screening of 150 isolates, mostly from Ecuadorian yeast biodiversity. The effect of nitrogen concentration was assessed in terms of cellular growth, glucose consumption and ethanol production, and the yeast strains’ dominance was evaluated in continuous co-fermentation with cellular recycling by mitochondrial DNA analyses. Among the four selected yeast strains under study, CLQCA-INT-005 presented the highest glucose consumption at a nitrogen supplement concentration as low as 0.4 g·L−1, attaining an ethanol yield of up to 96.72% in 24 h. The same yeast strain was found to be highly competitive, showing a dominance of 80% after four cycles of fermentation in co-culture. Thus, CLQCA-INT-005 may be deemed as a very promising candidate to be used both at pilot-plant scale and at industrial scale cellulosic ethanol production. 000079752 540__ $$9info:eu-repo/semantics/openAccess$$aby$$uhttp://creativecommons.org/licenses/by/3.0/es/ 000079752 590__ $$a2.702$$b2019 000079752 591__ $$aENERGY & FUELS$$b63 / 112 = 0.562$$c2019$$dQ3$$eT2 000079752 592__ $$a0.635$$b2019 000079752 593__ $$aControl and Optimization$$c2019$$dQ2 000079752 593__ $$aElectrical and Electronic Engineering$$c2019$$dQ2 000079752 593__ $$aRenewable Energy, Sustainability and the Environment$$c2019$$dQ2 000079752 593__ $$aEnergy Engineering and Power Technology$$c2019$$dQ2 000079752 593__ $$aFuel Technology$$c2019$$dQ2 000079752 593__ $$aEnergy (miscellaneous)$$c2019$$dQ2 000079752 655_4 $$ainfo:eu-repo/semantics/article$$vinfo:eu-repo/semantics/publishedVersion 000079752 700__ $$aMartín Gil, Jesús 000079752 700__ $$0(orcid)0000-0003-2713-2786$$aMartín Ramos, Pablo$$uUniversidad de Zaragoza 000079752 700__ $$aBriones Pérez, Ana 000079752 700__ $$aCarvajal Barriga, Enrique Javier 000079752 7102_ $$15011$$2500$$aUniversidad de Zaragoza$$bDpto. CC.Agrar.y Medio Natural$$cArea Ingeniería Agroforestal 000079752 773__ $$g12, 13 (2019), 2614 [12 pp.]$$pENERGIES$$tEnergies$$x1996-1073 000079752 8564_ $$s1590914$$uhttps://zaguan.unizar.es/record/79752/files/texto_completo.pdf$$yVersión publicada 000079752 8564_ $$s96247$$uhttps://zaguan.unizar.es/record/79752/files/texto_completo.jpg?subformat=icon$$xicon$$yVersión publicada 000079752 909CO $$ooai:zaguan.unizar.es:79752$$particulos$$pdriver 000079752 951__ $$a2020-07-16-09:09:11 000079752 980__ $$aARTICLE