000084156 001__ 84156 000084156 005__ 20200117212657.0 000084156 0247_ $$2doi$$a10.3168/jds.2018-14953 000084156 0248_ $$2sideral$$a108826 000084156 037__ $$aART-2018-108826 000084156 041__ $$aeng 000084156 100__ $$aSoler, L. 000084156 245__ $$aAcute-phase inter-alpha-trypsin inhibitor heavy chain 4 levels in serum and milk of cows with subclinical mastitis caused by Streptococcus species and coagulase-negative Staphylococcus species 000084156 260__ $$c2018 000084156 5060_ $$aAccess copy available to the general public$$fUnrestricted 000084156 5203_ $$aThe aim of the study was to investigate the concentrations of acute-phase inter-a-trypsin inhibitor heavy chain 4 (ITIH4) in serum and milk of cows with subclinical mastitis caused by Streptococcus sp. (STR) and coagulase-negative Staphylococcus sp. (CNS) and healthy cows. The blood and milk samples were obtained from 60 mid-lactation, multiparous Holstein-Friesian cows from 7 herds in the Lublin region of Poland. In the milk samples from 40 cows with subclinical mastitis, Streptococcus sp. and CNS were isolated. The ITIH4 was significantly higher in serum of cows with subclinical mastitis caused both by STR and CNS compared with healthy cows. One hundred percent of animals infected with Streptococcus sp. and 89% of animals infected with Staphylococcus sp. showed ITIH4 concentration in sera higher than 0.5 mg/mL. The concentration of ITIH4 in milk also was significantly higher in cows with subclinical mastitis caused by Streptococcus sp. and Staphylococcus sp. compared with the control group. Seventy percent of cows infected by STR and CNS showed ITIH4 concentration in milk higher than 2.5 µg/mL. Milk ITIH4 concentration higher than 5 µg/mL was found in 55% of animals infected with Streptococcus sp. and in 40% of animals infected with Staphylococcus sp. No statistically significant differences were observed in ITIH4 concentrations both in serum and in milk between the studied unhealthy animal groups. These results suggest that ITIH4 may be used in the future as a novel diagnostic marker in serum and in milk of subclinical mastitis in cows. 000084156 536__ $$9info:eu-repo/grantAgreement/ES/MINECO/RTC-2015-3885-2 000084156 540__ $$9info:eu-repo/semantics/openAccess$$aAll rights reserved$$uhttp://www.europeana.eu/rights/rr-f/ 000084156 590__ $$a3.082$$b2018 000084156 591__ $$aFOOD SCIENCE & TECHNOLOGY$$b33 / 135 = 0.244$$c2018$$dQ1$$eT1 000084156 591__ $$aAGRICULTURE, DAIRY & ANIMAL SCIENCE$$b4 / 61 = 0.066$$c2018$$dQ1$$eT1 000084156 592__ $$a1.34$$b2018 000084156 593__ $$aAnimal Science and Zoology$$c2018$$dQ1 000084156 593__ $$aGenetics$$c2018$$dQ1 000084156 593__ $$aFood Science$$c2018$$dQ1 000084156 655_4 $$ainfo:eu-repo/semantics/article$$vinfo:eu-repo/semantics/acceptedVersion 000084156 700__ $$aDabrowski, R. 000084156 700__ $$aGarcía, N. 000084156 700__ $$0(orcid)0000-0003-2872-4513$$aAlava, M.A.$$uUniversidad de Zaragoza 000084156 700__ $$0(orcid)0000-0002-3700-123X$$aLampreave, F.$$uUniversidad de Zaragoza 000084156 700__ $$aPiñeiro, M. 000084156 700__ $$aWawron, W. 000084156 700__ $$aSzczubial, M. 000084156 700__ $$aBochniarz, M. 000084156 7102_ $$11002$$2060$$aUniversidad de Zaragoza$$bDpto. Bioq.Biolog.Mol. Celular$$cÁrea Bioquímica y Biolog.Mole. 000084156 773__ $$g102 (2018), 1-8$$pJ. dairy sci.$$tJOURNAL OF DAIRY SCIENCE$$x0022-0302 000084156 8564_ $$s153492$$uhttps://zaguan.unizar.es/record/84156/files/texto_completo.pdf$$yPostprint 000084156 8564_ $$s125341$$uhttps://zaguan.unizar.es/record/84156/files/texto_completo.jpg?subformat=icon$$xicon$$yPostprint 000084156 909CO $$ooai:zaguan.unizar.es:84156$$particulos$$pdriver 000084156 951__ $$a2020-01-17-21:23:47 000084156 980__ $$aARTICLE