Acute-phase inter-alpha-trypsin inhibitor heavy chain 4 levels in serum and milk of cows with subclinical mastitis caused by Streptococcus species and coagulase-negative Staphylococcus species

Soler, L. ; Dabrowski, R. ; García, N. ; Alava, M.A. (Universidad de Zaragoza) ; Lampreave, F. (Universidad de Zaragoza) ; Piñeiro, M. ; Wawron, W. ; Szczubial, M. ; Bochniarz, M.

Resumen: The aim of the study was to investigate the concentrations of acute-phase inter-a-trypsin inhibitor heavy chain 4 (ITIH4) in serum and milk of cows with subclinical mastitis caused by Streptococcus sp. (STR) and coagulase-negative Staphylococcus sp. (CNS) and healthy cows. The blood and milk samples were obtained from 60 mid-lactation, multiparous Holstein-Friesian cows from 7 herds in the Lublin region of Poland. In the milk samples from 40 cows with subclinical mastitis, Streptococcus sp. and CNS were isolated. The ITIH4 was significantly higher in serum of cows with subclinical mastitis caused both by STR and CNS compared with healthy cows. One hundred percent of animals infected with Streptococcus sp. and 89% of animals infected with Staphylococcus sp. showed ITIH4 concentration in sera higher than 0.5 mg/mL. The concentration of ITIH4 in milk also was significantly higher in cows with subclinical mastitis caused by Streptococcus sp. and Staphylococcus sp. compared with the control group. Seventy percent of cows infected by STR and CNS showed ITIH4 concentration in milk higher than 2.5 µg/mL. Milk ITIH4 concentration higher than 5 µg/mL was found in 55% of animals infected with Streptococcus sp. and in 40% of animals infected with Staphylococcus sp. No statistically significant differences were observed in ITIH4 concentrations both in serum and in milk between the studied unhealthy animal groups. These results suggest that ITIH4 may be used in the future as a novel diagnostic marker in serum and in milk of subclinical mastitis in cows.

Idioma: Inglés

DOI: 10.3168/jds.2018-14953

Año: 2018

Publicado en: JOURNAL OF DAIRY SCIENCE 102 (2018), 1-8
ISSN: 0022-0302

Factor impacto JCR: 3.082 (2018)

Categ. JCR: FOOD SCIENCE & TECHNOLOGY rank: 33 / 135 = 0.244 (2018) - Q1 - T1
Categ. JCR: AGRICULTURE, DAIRY & ANIMAL SCIENCE rank: 4 / 61 = 0.066 (2018) - Q1 - T1

Factor impacto SCIMAGO: 1.34 - Animal Science and Zoology (Q1) - Genetics (Q1) - Food Science (Q1)

Financiación: info:eu-repo/grantAgreement/ES/MINECO/RTC-2015-3885-2

Tipo y forma: Article (PostPrint)

Área (Departamento): Área Bioquímica y Biolog.Mole. (Dpto. Bioq.Biolog.Mol. Celular)