84176 20191122145053.0 doi 10.1080/00218839.2018.1501856 sideral 108287 ART-2018-108287 eng Ángel Beamonte, Ernesto Automatic determination of landmark coordinates for honey bee forewing venation using a new MATLAB-based tool 2018 Access copy available to the general public Unrestricted In this study, a novel and free-to-use MATLAB-based tool (WingMarks) is presented, aimed at facilitating the geometric morphometric analysis of honey bee forewing venation through automatic recognition of vein junctions. Firstly, the ability of the new software to analyze wing images obtained by different optical equipment was evaluated. Even when a low-end USB-microscope was used for image collection, it reached 100% precision in the automatic detection of the landmarks for 87% of the samples, and most of failures corresponded to a single point and were easily corrected. The measurement error of WingMarks software was studied through repeated analysis of the same wing image, evincing that landmark determination was highly repeatable, even higher than that of widely used tpsDig manual software. In addition, a field test with 720 specimens from three subspecies (A. m. iberiensis, A. m. ligustica, and A. m. carnica) and from hybrid Buckfast bees, collected from 90 different colonies, was conducted. In conjunction with MorphoJ, the coordinates of the vein junctions extracted by the program led to an accurate classification of the specimens, confirmed by canonical variate analysis methods. WingMarks software can thus be deemed as a versatile, precise, and accurate tool for the automatic recognition of A. mellifera wing vein junctions, facilitating the identification of bee genetic diversity using geometric morphometrics. The program is available under Creative Commons license and can be easily adapted to different insect species. info:eu-repo/semantics/openAccess All rights reserved http://www.europeana.eu/rights/rr-f/ 1.752 2018 ENTOMOLOGY 31 / 98 = 0.316 2018 Q2 T1 0.626 2018 Insect Science 2018 Q2 info:eu-repo/semantics/article info:eu-repo/semantics/submittedVersion (orcid)0000-0003-2713-2786 Martín Ramos, Pablo Universidad de Zaragoza (orcid)0000-0001-8991-325X Santolaria, Pilar Universidad de Zaragoza (orcid)0000-0003-0582-5418 Sales, Ester Universidad de Zaragoza Abizanda, Javier Yániz, Jesús L. 5011 500 Universidad de Zaragoza Dpto. CC.Agrar.y Medio Natural Area Ingeniería Agroforestal 2008 700 Universidad de Zaragoza Dpto. Produc.Animal Cienc.Ali. Área Producción Animal 5011 705 Universidad de Zaragoza Dpto. CC.Agrar.y Medio Natural Área Producción Vegetal 57, 5 (2018), 605-610 J. Apic. Res. JOURNAL OF APICULTURAL RESEARCH 0021-8839 778504 http://zaguan.unizar.es/record/84176/files/texto_completo.pdf Preprint 39820 http://zaguan.unizar.es/record/84176/files/texto_completo.jpg?subformat=icon icon Preprint oai:zaguan.unizar.es:84176 articulos driver 2019-11-22-14:44:15 ARTICLE