84367 20200716101530.0 doi 10.3390/f10100885 sideral 113481 ART-2019-113481 eng Casado Sanz, María Milagrosa White-rot fungi control on Populus spp. Wood by pressure treatments with silver nanoparticles, chitosan oligomers and propolis 2019 Access copy available to the general public Unrestricted There is growing interest in the development of non-toxic, natural wood preservation agents to replace conventional chemicals. In this paper, the antifungal activities of silver nanoparticles, chitosan oligomers, and propolis ethanolic extract were evaluated against white-rot fungus Trametes versicolor (L.) Lloyd, with a view to protecting Populus spp. wood. In order to create a more realistic in-service type environment, the biocidal products were assessed according to EN:113 European standard, instead of using routine in vitro antimicrobial susceptibility testing methods. Wood blocks were impregnated with the aforementioned antifungal agents by the vacuum-pressure method in an autoclave, and their biodeterioration was monitored over 16 weeks. The results showed that treatments based on silver nanoparticles, at concentrations ranging from 5 to 20 ppm, presented high antifungal activity, protecting the wood from fungal attack over time, with weight losses in the range of 8.49% to 8.94% after 16 weeks, versus 24.79% weight loss in the control (untreated) samples. This was confirmed by SEM and optical microscopy images, which showed a noticeably higher cell wall degradation in control samples than in samples treated with silver nanoparticles. On the other hand, the efficacy of the treatments based on chitosan oligomers and propolis gradually decreased over time, which would be a limiting factor for their application as wood preservatives. The nanometal-based approach is thus posed as the preferred choice for the industrial treatment of poplar wood aimed at wood-based engineering products (plywood, laminated veneer lumber, cross-laminated timber, etc.). info:eu-repo/grantAgreement/ES/UZ/UZ2019-TEC-07 info:eu-repo/semantics/openAccess by http://creativecommons.org/licenses/by/3.0/es/ 2.221 2019 FORESTRY 17 / 68 = 0.25 2019 Q1 T1 0.652 2019 Forestry 2019 Q1 info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion Silva Castro, Iosody Ponce Herrero, Laura (orcid)0000-0003-2713-2786 Martín Ramos, Pablo Universidad de Zaragoza Martín Gil, Jesús Acuña Rello, Luis 5011 500 Universidad de Zaragoza Dpto. CC.Agrar.y Medio Natural Area Ingeniería Agroforestal 10, 10 (2019), 885 [10 pp.] Forests Forests 1999-4907 1278930 http://zaguan.unizar.es/record/84367/files/texto_completo.pdf Versión publicada 94657 http://zaguan.unizar.es/record/84367/files/texto_completo.jpg?subformat=icon icon Versión publicada oai:zaguan.unizar.es:84367 articulos driver 2020-07-16-09:33:37 ARTICLE