000084714 001__ 84714
000084714 005__ 20200716101537.0
000084714 0247_ $$2doi$$a10.3389/fcimb.2019.00298
000084714 0248_ $$2sideral$$a114029
000084714 037__ $$aART-2019-114029
000084714 041__ $$aeng
000084714 100__ $$aObregón, Dasiel
000084714 245__ $$aSex-Specific Linkages Between Taxonomic and Functional Profiles of Tick Gut Microbiomes
000084714 260__ $$c2019
000084714 5060_ $$aAccess copy available to the general public$$fUnrestricted
000084714 5203_ $$aTicks transmit the most diverse array of disease agents and harbor one of the most diverse microbial communities. Major progress has been made in the characterization of the taxonomic profiles of tick microbiota. However, the functional profiles of tick microbiome have been comparatively less studied. In this proof of concept we used state-of-the-art functional metagenomics analytical tools to explore previously reported datasets of bacteria found in male and female Ixodes ovatus, Ixodes persulcatus, and Amblyomma variegatum. Results showed that both taxonomic and functional profiles have differences between sexes of the same species. KEGG pathway analysis revealed that male and female of the same species had major differences in the abundance of genes involved in different metabolic pathways including vitamin B, amino acids, carbohydrates, nucleotides, and antibiotics among others. Partial reconstruction of metabolic pathways using KEGG enzymes suggests that tick microbiome form a complex metabolic network that may increase microbial community resilience and adaptability. Linkage analysis between taxonomic and functional profiles showed that among the KEGG enzymes with differential abundance in male and female ticks only 12% were present in single bacterial genera. The rest of these enzymes were found in more than two bacterial genera, and 27% of them were found in five up to ten bacterial genera. Comparison of bacterial genera contributing to the differences in the taxonomic and functional profiles of males and females revealed that while a small group of bacteria has a dual-role, most of the bacteria contribute only to functional or taxonomic differentiation between sexes. Results suggest that the different life styles of male and female ticks exert sex-specific evolutionary pressures that act independently on the phenomes (set of phenotypes) and genomes of bacteria in tick gut microbiota. We conclude that functional redundancy is a fundamental property of male and female tick microbiota and propose that functional metagenomics should be combined with taxonomic profiling of microbiota because both analyses are complementary.
000084714 540__ $$9info:eu-repo/semantics/openAccess$$aby$$uhttp://creativecommons.org/licenses/by/3.0/es/
000084714 590__ $$a4.123$$b2019
000084714 591__ $$aMICROBIOLOGY$$b38 / 134 = 0.284$$c2019$$dQ2$$eT1
000084714 591__ $$aIMMUNOLOGY$$b55 / 158 = 0.348$$c2019$$dQ2$$eT2
000084714 592__ $$a1.626$$b2019
000084714 593__ $$aImmunology$$c2019$$dQ1
000084714 593__ $$aInfectious Diseases$$c2019$$dQ1
000084714 593__ $$aMicrobiology (medical)$$c2019$$dQ1
000084714 593__ $$aMicrobiology$$c2019$$dQ1
000084714 593__ $$aMedicine (miscellaneous)$$c2019$$dQ1
000084714 655_4 $$ainfo:eu-repo/semantics/article$$vinfo:eu-repo/semantics/publishedVersion
000084714 700__ $$aBard, Emilie
000084714 700__ $$aAbrial, David
000084714 700__ $$0(orcid)0000-0001-7483-046X$$aEstrada-Peña, Agustín$$uUniversidad de Zaragoza
000084714 700__ $$aCabezas-Cruz, Alejandro
000084714 7102_ $$11009$$2773$$aUniversidad de Zaragoza$$bDpto. Patología Animal$$cÁrea Sanidad Animal
000084714 773__ $$g9 (2019), 298 [16 pp.]$$tFrontiers in cellular and infection microbiology$$x2235-2988
000084714 8564_ $$s4959049$$uhttps://zaguan.unizar.es/record/84714/files/texto_completo.pdf$$yVersión publicada
000084714 8564_ $$s12113$$uhttps://zaguan.unizar.es/record/84714/files/texto_completo.jpg?subformat=icon$$xicon$$yVersión publicada
000084714 909CO $$ooai:zaguan.unizar.es:84714$$particulos$$pdriver
000084714 951__ $$a2020-07-16-09:37:40
000084714 980__ $$aARTICLE