000086124 001__ 86124
000086124 005__ 20200716101446.0
000086124 0247_ $$2doi$$a10.1007/978-1-4939-8885-3_13
000086124 0248_ $$2sideral$$a109725
000086124 037__ $$aART-2019-109725
000086124 041__ $$aeng
000086124 100__ $$0(orcid)0000-0002-1861-5981$$aSantiago, L.$$uUniversidad de Zaragoza
000086124 245__ $$aMouse model of colitis-associated colorectal cancer (CAC): Isolation and characterization of mucosal-associated lymphoid cells
000086124 260__ $$c2019
000086124 5060_ $$aAccess copy available to the general public$$fUnrestricted
000086124 5203_ $$aColorectal cancer (CRC) is the third most common malignancy worldwide presenting high mortality due to low treatment efficacy. Existing evidence indicates that inflammatory bowel disease (IBD) is associated with a higher risk of developing CRC. Many murine models of inflammation-related colon carcinogenesis (CAC) have been developed to study colon carcinogenesis and novel treatments. A commonly used model involves the combination of a single dose of azoxymethane (AOM), together with three cycles of the inflammatory agent dextran sodium sulfate (DSS) (5 days in drinking water followed by a two-week rest). Following this protocol, around 50% of the animals develop CRCs after 45 days and almost 100% of animals after 60 days. During CAC development, immune cells, cytokines, and other immune mediators are involved in both tumorigenesis and the elimination of cancer cells during immunotherapy. Thus, the study of mucosal immune responses (including lamina propria mononuclear cells and intraepithelial lymphocytes) is important to understand the role of the immune system during development and therapy in CRC. Single immune cell suspensions from lamina propria and epithelium can be purified combining selective tissue digestion and Percoll gradient centrifugation. Isolated cells can be characterized using flow cytometry by analyzing surface antigens or intracellular cytokines and cytotoxic mediators or employed for further investigations like comparative studies of mRNA expression, cell-proliferation assay, protein analysis, or even functional cytotoxicity assays. The CAC model is useful to study the involvement of immune cells not only during the carcinogenesis process but, in addition, during the treatment with novel immunotherapy protocols.
000086124 536__ $$9info:eu-repo/grantAgreement/ES/MINECO/AEI-010500-2015-161$$9info:eu-repo/grantAgreement/ES/MINECO/SAF2014-54763-C2-1-R
000086124 540__ $$9info:eu-repo/semantics/openAccess$$aAll rights reserved$$uhttp://www.europeana.eu/rights/rr-f/
000086124 592__ $$a0.597$$b2019
000086124 593__ $$aMolecular Biology$$c2019$$dQ3
000086124 593__ $$aGenetics$$c2019$$dQ3
000086124 655_4 $$ainfo:eu-repo/semantics/article$$vinfo:eu-repo/semantics/acceptedVersion
000086124 700__ $$0(orcid)0000-0001-8584-3979$$aCastro, M.$$uUniversidad de Zaragoza
000086124 700__ $$0(orcid)0000-0003-0154-0730$$aPardo, J.$$uUniversidad de Zaragoza
000086124 700__ $$0(orcid)0000-0002-9730-2210$$aArias, M.
000086124 7102_ $$11005$$2410$$aUniversidad de Zaragoza$$bDpto. Farmacología y Fisiolog.$$cÁrea Fisiología
000086124 7102_ $$11008$$2566$$aUniversidad de Zaragoza$$bDpto. Microb.Med.Pr.,Sal.Públ.$$cÁrea Inmunología
000086124 773__ $$g1884 (2019), 189-202$$pMethods mol. biol. (Clifton N.J.)$$tMethods in Molecular Biology$$x1064-3745
000086124 8564_ $$s691674$$uhttps://zaguan.unizar.es/record/86124/files/texto_completo.pdf$$yPostprint
000086124 8564_ $$s16187$$uhttps://zaguan.unizar.es/record/86124/files/texto_completo.jpg?subformat=icon$$xicon$$yPostprint
000086124 909CO $$ooai:zaguan.unizar.es:86124$$particulos$$pdriver
000086124 951__ $$a2020-07-16-09:03:23
000086124 980__ $$aARTICLE