000086204 001__ 86204
000086204 005__ 20231006143256.0
000086204 0247_ $$2doi$$a10.32794/mr11250017
000086204 0248_ $$2sideral$$a114908
000086204 037__ $$aART-2019-114908
000086204 041__ $$aeng
000086204 100__ $$0(orcid)0000-0002-2323-2053$$aReyes-Gonzales, M.$$uUniversidad de Zaragoza
000086204 245__ $$aAntioxidant activity of pineal methoxyindoles on hepatocyte plasmatic membrane
000086204 260__ $$c2019
000086204 5060_ $$aAccess copy available to the general public$$fUnrestricted
000086204 5203_ $$aAntioxidant effect of several pineal derived molecules has been well documented. Here, the protective effects of 5-methoxytryptophol (5-MTOH) and 5-methoxyindol-3-acetic acid (5-MIAA) on hepatic cell membrane lipid peroxidation and cell membrane rigidity induced by FeCl3 plus ascorbic acid have been systemically investigated. The membrane fluidity was evaluated by fluorescence spectroscopy, malondialdehyde (MDA) and 4-hydroxyalkenals (4-HDA) concentrations and carbonyl groups of protein were measured as the parameters of lipid and protein damage, respectively. Results showed that oxidative stress increased membrane rigidity, MDA and 4-HDA concentra-tions as well as carbonyl content in a concentration-dependent manner. 5-MTOH, but not 5-MIAA, significantly attenuated these oxidative indecies. In absence of oxidative stress, none of these methoxyindoleamines modified the content of MDA, 4-HDA or carbonyl-ation. However 5-MIAA at its highest concentration slightly modified membrane fluidity. The results suggest that structural modification of C3 in the methoxyindoleamine, that is, the carboxyl group replaced by hydroxyl group in this site could improve the ability of 5-methoxyindoleamine derivatives to preserve membrane fluidity of cells which are under oxidative stress.
000086204 536__ $$9info:eu-repo/grantAgreement/ES/DGA/B37-17D
000086204 540__ $$9info:eu-repo/semantics/openAccess$$aby$$uhttp://creativecommons.org/licenses/by/3.0/es/
000086204 655_4 $$ainfo:eu-repo/semantics/article$$vinfo:eu-repo/semantics/publishedVersion
000086204 700__ $$0(orcid)0000-0003-3957-3749$$aEsteban-Zubero, E.$$uUniversidad de Zaragoza
000086204 700__ $$0(orcid)0000-0002-2455-680X$$aLópez-Pingarrón, L.$$uUniversidad de Zaragoza
000086204 700__ $$0(orcid)0000-0002-6001-2738$$aSoria, M.$$uUniversidad de Zaragoza
000086204 700__ $$0(orcid)0000-0002-9357-143X$$aPereboom, D.$$uUniversidad de Zaragoza
000086204 700__ $$aTan, D.
000086204 700__ $$aReiter, R.
000086204 700__ $$0(orcid)0000-0001-9507-6478$$aGarcía, J.$$uUniversidad de Zaragoza
000086204 7102_ $$11005$$2410$$aUniversidad de Zaragoza$$bDpto. Farmacología y Fisiolog.$$cÁrea Fisiología
000086204 773__ $$g2, 1 (2019), 161-174$$pMelatonin res.$$tMelatonin research$$x2641-0281
000086204 8564_ $$s573399$$uhttps://zaguan.unizar.es/record/86204/files/texto_completo.pdf$$yVersión publicada
000086204 8564_ $$s110770$$uhttps://zaguan.unizar.es/record/86204/files/texto_completo.jpg?subformat=icon$$xicon$$yVersión publicada
000086204 909CO $$ooai:zaguan.unizar.es:86204$$particulos$$pdriver
000086204 951__ $$a2023-10-06-14:06:16
000086204 980__ $$aARTICLE