000086223 001__ 86223
000086223 005__ 20191128095712.0
000086223 0247_ $$2doi$$a10.1021/acsami.8b18270
000086223 0248_ $$2sideral$$a109607
000086223 037__ $$aART-2018-109607
000086223 041__ $$aeng
000086223 100__ $$0(orcid)0000-0003-4516-8694$$aBeola, L.
000086223 245__ $$aDual Role of Magnetic Nanoparticles as Intracellular Hotspots and Extracellular Matrix Disruptors Triggered by Magnetic Hyperthermia in 3D Cell Culture Models
000086223 260__ $$c2018
000086223 5060_ $$aAccess copy available to the general public$$fUnrestricted
000086223 5203_ $$aMagnetic hyperthermia is a promising therapy for the localized treatment of cancer based on the exposure of magnetic nanoparticles to an external alternating magnetic field. In order to evaluate some of the mechanisms involved in the cellular damage caused by this treatment, two different 3D cell culture models were prepared using collagen, which is the most abundant protein of the extracellular matrix. The same amount of nanoparticles was added to cells either before or after their incorporation into the 3D structure. Therefore, in one model, particles were located only inside cells (In model), while the other one had particles both inside and outside cells (In&Out model). In the In&Out model, the hyperthermia treatment facilitated the migration of the particles from the outer areas of the 3D structure to the inner parts, achieving a faster homogeneous distribution throughout the whole structure and allowing the particles to gain access to the inner cells. The cell death mechanism activated by the magnetic hyperthermia treatment was different in both models. Necrosis was observed in the In model and apoptosis in the In&Out model 24 h after the hyperthermia application. This was clearly correlated with the amount of nanoparticles located inside the cells. Thus, the combination of both 3D models allowed us to demonstrate two different roles of the magnetic particles during the hyperthermia treatment: (i) The modulation of the cell death mechanism depending on the amount of intracellular particles and (ii) the disruption of the collagen matrix caused by the extracellular nanoparticles.
000086223 536__ $$9info:eu-repo/grantAgreement/EUR/COST-Action/TD1402-Radiomag$$9info:eu-repo/grantAgreement/ES/DGA/FSE$$9info:eu-repo/grantAgreement/EUR/M-ERA.NET/COFUND-MagicCellGene-PCIN-2017-060$$9info:eu-repo/grantAgreement/ES/MINECO/BIO2017-84246-C2-1-R$$9info:eu-repo/grantAgreement/ES/MINECO/MAT2011-26851-CO2-01$$9info:eu-repo/grantAgreement/ES/MINECO/RYC-2014-15512$$9info:eu-repo/grantAgreement/ES/MINECO/RYC-2015-17640$$9info:eu-repo/grantAgreement/ES/MINECO/SAF2014-54763-C2-2-R$$9info:eu-repo/grantAgreement/ES/UZ/UZ2018-CIE-03
000086223 540__ $$9info:eu-repo/semantics/openAccess$$aAll rights reserved$$uhttp://www.europeana.eu/rights/rr-f/
000086223 590__ $$a8.456$$b2018
000086223 591__ $$aNANOSCIENCE & NANOTECHNOLOGY$$b16 / 94 = 0.17$$c2018$$dQ1$$eT1
000086223 591__ $$aMATERIALS SCIENCE, MULTIDISCIPLINARY$$b27 / 293 = 0.092$$c2018$$dQ1$$eT1
000086223 592__ $$a2.596$$b2018
000086223 593__ $$aMaterials Science (miscellaneous)$$c2018$$dQ1
000086223 593__ $$aNanoscience and Nanotechnology$$c2018$$dQ1
000086223 593__ $$aMedicine (miscellaneous)$$c2018$$dQ1
000086223 655_4 $$ainfo:eu-repo/semantics/article$$vinfo:eu-repo/semantics/acceptedVersion
000086223 700__ $$0(orcid)0000-0003-0641-3407$$aAsín, L.$$uUniversidad de Zaragoza
000086223 700__ $$0(orcid)0000-0001-5559-8757$$aFratila, R.M.
000086223 700__ $$aHerrero, V.
000086223 700__ $$0(orcid)0000-0003-1081-8482$$aDe La Fuente, J.M.$$uUniversidad de Zaragoza
000086223 700__ $$0(orcid)0000-0001-6170-4237$$aGrazú, V.$$uUniversidad de Zaragoza
000086223 700__ $$0(orcid)0000-0003-2366-3598$$aGutiérrez, L.$$uUniversidad de Zaragoza
000086223 7102_ $$12009$$2750$$aUniversidad de Zaragoza$$bDpto. Química Analítica$$cÁrea Química Analítica
000086223 7102_ $$12013$$2765$$aUniversidad de Zaragoza$$bDpto. Química Orgánica$$cÁrea Química Orgánica
000086223 773__ $$g10, 51 (2018), 44301-44313$$pACS appl. mater. interfaces$$tACS Applied Materials & Interfaces$$x1944-8244
000086223 8564_ $$s1501468$$uhttps://zaguan.unizar.es/record/86223/files/texto_completo.pdf$$yPostprint
000086223 8564_ $$s368944$$uhttps://zaguan.unizar.es/record/86223/files/texto_completo.jpg?subformat=icon$$xicon$$yPostprint
000086223 909CO $$ooai:zaguan.unizar.es:86223$$particulos$$pdriver
000086223 951__ $$a2019-11-27-15:43:55
000086223 980__ $$aARTICLE