86223 20191128095712.0 doi 10.1021/acsami.8b18270 sideral 109607 ART-2018-109607 eng (orcid)0000-0003-4516-8694 Beola, L. Dual Role of Magnetic Nanoparticles as Intracellular Hotspots and Extracellular Matrix Disruptors Triggered by Magnetic Hyperthermia in 3D Cell Culture Models 2018 Access copy available to the general public Unrestricted Magnetic hyperthermia is a promising therapy for the localized treatment of cancer based on the exposure of magnetic nanoparticles to an external alternating magnetic field. In order to evaluate some of the mechanisms involved in the cellular damage caused by this treatment, two different 3D cell culture models were prepared using collagen, which is the most abundant protein of the extracellular matrix. The same amount of nanoparticles was added to cells either before or after their incorporation into the 3D structure. Therefore, in one model, particles were located only inside cells (In model), while the other one had particles both inside and outside cells (In&Out model). In the In&Out model, the hyperthermia treatment facilitated the migration of the particles from the outer areas of the 3D structure to the inner parts, achieving a faster homogeneous distribution throughout the whole structure and allowing the particles to gain access to the inner cells. The cell death mechanism activated by the magnetic hyperthermia treatment was different in both models. Necrosis was observed in the In model and apoptosis in the In&Out model 24 h after the hyperthermia application. This was clearly correlated with the amount of nanoparticles located inside the cells. Thus, the combination of both 3D models allowed us to demonstrate two different roles of the magnetic particles during the hyperthermia treatment: (i) The modulation of the cell death mechanism depending on the amount of intracellular particles and (ii) the disruption of the collagen matrix caused by the extracellular nanoparticles. info:eu-repo/grantAgreement/EUR/COST-Action/TD1402-Radiomag info:eu-repo/grantAgreement/ES/DGA/FSE info:eu-repo/grantAgreement/EUR/M-ERA.NET/COFUND-MagicCellGene-PCIN-2017-060 info:eu-repo/grantAgreement/ES/MINECO/BIO2017-84246-C2-1-R info:eu-repo/grantAgreement/ES/MINECO/MAT2011-26851-CO2-01 info:eu-repo/grantAgreement/ES/MINECO/RYC-2014-15512 info:eu-repo/grantAgreement/ES/MINECO/RYC-2015-17640 info:eu-repo/grantAgreement/ES/MINECO/SAF2014-54763-C2-2-R info:eu-repo/grantAgreement/ES/UZ/UZ2018-CIE-03 info:eu-repo/semantics/openAccess All rights reserved http://www.europeana.eu/rights/rr-f/ 8.456 2018 NANOSCIENCE & NANOTECHNOLOGY 16 / 94 = 0.17 2018 Q1 T1 MATERIALS SCIENCE, MULTIDISCIPLINARY 27 / 293 = 0.092 2018 Q1 T1 2.596 2018 Materials Science (miscellaneous) 2018 Q1 Nanoscience and Nanotechnology 2018 Q1 Medicine (miscellaneous) 2018 Q1 info:eu-repo/semantics/article info:eu-repo/semantics/acceptedVersion (orcid)0000-0003-0641-3407 Asín, L. Universidad de Zaragoza (orcid)0000-0001-5559-8757 Fratila, R.M. Herrero, V. (orcid)0000-0003-1081-8482 De La Fuente, J.M. Universidad de Zaragoza (orcid)0000-0001-6170-4237 Grazú, V. Universidad de Zaragoza (orcid)0000-0003-2366-3598 Gutiérrez, L. Universidad de Zaragoza 2009 750 Universidad de Zaragoza Dpto. Química Analítica Área Química Analítica 2013 765 Universidad de Zaragoza Dpto. Química Orgánica Área Química Orgánica 10, 51 (2018), 44301-44313 ACS appl. mater. interfaces ACS Applied Materials & Interfaces 1944-8244 1501468 http://zaguan.unizar.es/record/86223/files/texto_completo.pdf Postprint 368944 http://zaguan.unizar.es/record/86223/files/texto_completo.jpg?subformat=icon icon Postprint oai:zaguan.unizar.es:86223 articulos driver 2019-11-27-15:43:55 ARTICLE