000086821 001__ 86821 000086821 005__ 20200108151738.0 000086821 037__ $$aTAZ-TFM-2019-344 000086821 041__ $$aeng 000086821 1001_ $$aLópez Borbón, Adrian 000086821 24200 $$aStudy of the structure-propensities of alpha-synuclein sequence 000086821 24500 $$aEstudio de las propensidades estructurales de la sequencia de alfa sinucleína 000086821 260__ $$aZaragoza$$bUniversidad de Zaragoza$$c2019 000086821 506__ $$aby-nc-sa$$bCreative Commons$$c3.0$$uhttp://creativecommons.org/licenses/by-nc-sa/3.0/ 000086821 520__ $$aThe amyloid aggregation of alpha synuclein (αS), an intrinsically disordered protein, plays a key role in the etiology of Parkinson disease. The molecular mechanisms of αS amyloid aggregation are not well described at the moment and in this research, we move the first steps towards the characterization of two different mechanisms of αS aggregation as well as the of the phase transition between them using methanol (MetOH) as an inducing agent, with an approach that will combine the experimental and theoretical perspective with a residues-based theoretical modelling.<br />On the one hand the determination of the phase transition range between the two observed mechanisms was carried out by performing αS aggregation kinetics at different MetOH concentrations and carrying out structural analysis of the generated aggregates by Fourier Transform Infrared Spectroscopy (FTIR). On the other hand, the preference for one mechanism or the other was studied with pyrene excimer emission fluorescence spectroscopy and the coexistence of both types of aggregation mechanisms was observed in the transition phase. The results obtained from FTIR and emission fluorescence spectroscopy were in agreement and complementary to each other.<br />From the theoretical perspective an initial approach for the αS aggregation modelling was given by computing the accuracy of online secondary structure predictors in order to determine the importance of different αS amino acid regions in the preference for the type of amyloid aggregation mechanism. <br /><br /> 000086821 521__ $$aMáster Universitario en Biotecnología Cuantitativa 000086821 540__ $$aDerechos regulados por licencia Creative Commons 000086821 700__ $$aCremades Casasín, Nunilo $$edir. 000086821 700__ $$aBruscolini, Pierpaolo$$edir. 000086821 7102_ $$aUniversidad de Zaragoza$$bFísica Teórica$$cFísica Teórica 000086821 8560_ $$f792330@celes.unizar.es 000086821 8564_ $$s2124686$$uhttps://zaguan.unizar.es/record/86821/files/TAZ-TFM-2019-344.pdf$$yMemoria (eng) 000086821 909CO $$ooai:zaguan.unizar.es:86821$$pdriver$$ptrabajos-fin-master 000086821 950__ $$a 000086821 951__ $$adeposita:2020-01-08 000086821 980__ $$aTAZ$$bTFM$$cCIEN 000086821 999__ $$a20190628135735.CREATION_DATE