87548 20200716101515.0 doi 10.1016/j.cryobiol.2019.01.009 sideral 110408 ART-2019-110408 eng Álvarez, C. A preliminary study on the use of jenny colostrum to improve quality in extenders for freezing donkey semen 2019 Access copy available to the general public Unrestricted Sperm quality in donkeys (Equus asinus) after freezing thawing is still considered lower than that from other animals, including horses. The aim of this study was to test a new freezing extender supplemented with jenny colostrum on donkey sperm. After thawing, we evaluated sperm motility by means of computer-assisted analysis, viability by SYBR-14 and propidium iodide (PI), membrane functional integrity by HOS-test and acrosome integrity by isothiocyanate conjugated with peanut agglutinin (FITC-PNA) and PI. Ejaculates were collected from five fertile Donkeys. Sperm samples were pooled, diluted and cryopreserved into three experimental extender groups: BotuCrio®, lactose-extender supplemented with egg yolk (20%) and lactose-extender supplemented with jenny colostrum (20%). The results demonstrated that lactose-jenny colostrum samples displayed significantly higher values in almost all parameters evaluated (p < 0.05) compared with the other two extenders after thawing (BotuCrio® and lactose-egg yolk based extender, respectively) –Total Motility, Viability, HOS test, VCL, VSL and VAP. Acrosome status, LIN, STR and WOB despite showing lower values, none of them were statistically significant (p > 0.05). In conclusion, the extender containing jenny colostrum can be successfully used for donkey semen crypreservation and could effectively improve donkey sperm qualities after freezing -thawing. info:eu-repo/grantAgreement/ES/DGA/FSE info:eu-repo/semantics/openAccess All rights reserved http://www.europeana.eu/rights/rr-f/ 2.283 2019 BIOLOGY 40 / 93 = 0.43 2019 Q2 T2 PHYSIOLOGY 44 / 81 = 0.543 2019 Q3 T2 0.696 2019 Agricultural and Biological Sciences (miscellaneous) 2019 Q1 Medicine (miscellaneous) 2019 Q2 Biochemistry, Genetics and Molecular Biology (miscellaneous) 2019 Q2 info:eu-repo/semantics/article info:eu-repo/semantics/acceptedVersion (orcid)0000-0002-7857-9732 Luño, V. Universidad de Zaragoza (orcid)0000-0002-1611-6337 González, N. Universidad de Zaragoza (orcid)0000-0002-0797-1841 Gil, L. Universidad de Zaragoza 1009 617 Universidad de Zaragoza Dpto. Patología Animal Área Medicina y Cirugía Animal 87 (2019), 110-114 Cryobiology Cryobiology 0011-2240 163702 http://zaguan.unizar.es/record/87548/files/texto_completo.pdf Postprint 42624 http://zaguan.unizar.es/record/87548/files/texto_completo.jpg?subformat=icon icon Postprint oai:zaguan.unizar.es:87548 articulos driver 2020-07-16-09:22:46 ARTICLE