000087550 001__ 87550
000087550 005__ 20200716101516.0
000087550 0247_ $$2doi$$a10.1016/j.fm.2019.01.010
000087550 0248_ $$2sideral$$a109895
000087550 037__ $$aART-2019-109895
000087550 041__ $$aeng
000087550 100__ $$0(orcid)0000-0003-1053-2556$$aCalvo Crespo, Hector$$uUniversidad de Zaragoza
000087550 245__ $$aThe role of iturin A from B. amyloliquefaciens BUZ-14 in the inhibition of the most common postharvest fruit rots
000087550 260__ $$c2019
000087550 5060_ $$aAccess copy available to the general public$$fUnrestricted
000087550 5203_ $$aThe aim of this work was to elucidate the role of the secondary metabolites produced by B. amyloliquefaciens BUZ-14 against B. cinerea, M. fructicola, M. laxa, P. digitatum, P. italicum and P. expansum both in vitro and in planta. The entire cell free supernatant (CFS) and the lipopeptide fraction (LPF) showed similar antifungal activities, completely inhibiting all the fungi at dilutions of 1:24 or even lower, whereas the non-butanolic fraction (NBF) barely inhibited the fungi. However, when the LPF and CFS were applied on fruit, only brown rot in peaches and blue rot in apples was totally inhibited. The main families of metabolites in the LPF were iturin A, fengycin and surfactin with maximum concentrations of 407, 853 and 658¿µg¿mL-1, respectively. Subsequently, a TLC-bioautography revealed iturin A as the key metabolite in the inhibitions and allowed us to establish in vivo MICs of 16.9 and 33.9¿µg¿mL-1 for Monilinia species and P. expansum, respectively. The application of 24 h-old BUZ-14 cultures suppressed brown rot in peaches and also blue rot in apples but failed to inhibit the other diseases. However, BUZ-14 was only able to grow and produce iturin A in peaches so we can deduce that the amount of iturin A brought with the cultures (36¿±¿14¿µg¿mL-1) could be enough to control both diseases. The strong antifungal activity of the iturin A present in the BUZ-14 CFS suggests that it could be successfully used for postharvest disease control. However, future research is necessary to maximize the iturin A production by B. amyloliquefaciens BUZ-14 in order to optimize a commercial application.
000087550 536__ $$9info:eu-repo/grantAgreement/ES/DGA/T07-17R$$9info:eu-repo/grantAgreement/ES/MINECO/RTC-2015--4121-2
000087550 540__ $$9info:eu-repo/semantics/openAccess$$aby-nc-nd$$uhttp://creativecommons.org/licenses/by-nc-nd/3.0/es/
000087550 590__ $$a4.155$$b2019
000087550 591__ $$aBIOTECHNOLOGY & APPLIED MICROBIOLOGY$$b33 / 156 = 0.212$$c2019$$dQ1$$eT1
000087550 591__ $$aFOOD SCIENCE & TECHNOLOGY$$b25 / 139 = 0.18$$c2019$$dQ1$$eT1
000087550 591__ $$aMICROBIOLOGY$$b36 / 134 = 0.269$$c2019$$dQ2$$eT1
000087550 592__ $$a1.318$$b2019
000087550 593__ $$aFood Science$$c2019$$dQ1
000087550 593__ $$aMicrobiology$$c2019$$dQ2
000087550 655_4 $$ainfo:eu-repo/semantics/article$$vinfo:eu-repo/semantics/acceptedVersion
000087550 700__ $$0(orcid)0000-0001-8830-6554$$aMendiara Negredo, Isabel$$uUniversidad de Zaragoza
000087550 700__ $$0(orcid)0000-0002-4843-2561$$aArias Alvarez, Esther$$uUniversidad de Zaragoza
000087550 700__ $$0(orcid)0000-0001-6739-5375$$aBlanco Parmo, Domingo$$uUniversidad de Zaragoza
000087550 700__ $$0(orcid)0000-0001-6316-385X$$aVenturini Crespo, Maria Eugenia$$uUniversidad de Zaragoza
000087550 7102_ $$12008$$2640$$aUniversidad de Zaragoza$$bDpto. Produc.Animal Cienc.Ali.$$cÁrea Nutrición Bromatología
000087550 7102_ $$12008$$2780$$aUniversidad de Zaragoza$$bDpto. Produc.Animal Cienc.Ali.$$cÁrea Tecnología de Alimentos
000087550 773__ $$g82 (2019), 62-69$$pFood microbiol.$$tFOOD MICROBIOLOGY$$x0740-0020
000087550 8564_ $$s302804$$uhttps://zaguan.unizar.es/record/87550/files/texto_completo.pdf$$yPostprint
000087550 8564_ $$s180745$$uhttps://zaguan.unizar.es/record/87550/files/texto_completo.jpg?subformat=icon$$xicon$$yPostprint
000087550 909CO $$ooai:zaguan.unizar.es:87550$$particulos$$pdriver
000087550 951__ $$a2020-07-16-09:23:20
000087550 980__ $$aARTICLE