000087566 001__ 87566
000087566 005__ 20230921135430.0
000087566 0247_ $$2doi$$a10.1038/s41598-019-56547-z
000087566 0248_ $$2sideral$$a115459
000087566 037__ $$aART-2019-115459
000087566 041__ $$aeng
000087566 100__ $$0(orcid)0000-0003-3660-8651$$aVirumbrales Muñoz, María
000087566 245__ $$aForce spectroscopy-based simultaneous topographical and mechanical characterization to study polymer-to-polymer interactions in coated alginate microspheres
000087566 260__ $$c2019
000087566 5060_ $$aAccess copy available to the general public$$fUnrestricted
000087566 5203_ $$aCell-laden hydrogel microspheres have shown encouraging outcomes in the fields of drug delivery, tissue engineering or regenerative medicine. Beyond the classical single coating with polycations, many other different coating designs have been reported with the aim of improving mechanical properties and in vivo performance of the microspheres. Among the most common strategies are the inclusion of additional polycation coatings and the covalent bonding of the semi-permeable membranes with biocompatible crosslinkers such as genipin. However, it remains challenging to characterize the effects of the interactions between the polycations and the hydrogel microspheres over time in vitro. Here we use a force spectroscopy-based simultaneous topographical and mechanical characterization to study polymer-to-polymer interactions in alginate microspheres with different coating designs, maintaining the hydrogels in liquid. In addition to classical topography parameters, we explored, for the first time, the evolution of peak/valley features along the z axis via thresholding analysis and the cross-correlation between topography and stiffness profiles with resolution down to tens of nanometers. Thus, we demonstrated the importance of genipin crosslinking to avoid membrane detachment in alginate microspheres with double polycation coatings. Overall, this methodology could improve hydrogel design rationale and expedite in vitro characterization, therefore facilitating clinical translation of hydrogel-based technologies.
000087566 536__ $$9info:eu-repo/grantAgreement/ES/DGA/T24-17R$$9info:eu-repo/grantAgreement/EC/H2020/783132/EU/A pilot line for the next generation of smart catheters and implants/POSITION-II$$9This project has received funding from the European Union’s Horizon 2020 research and innovation program under grant agreement No H2020 783132-POSITION-II$$9info:eu-repo/grantAgreement/ES/MICINN/MTM2012-31073$$9info:eu-repo/grantAgreement/ES/MINECO/BIO2016-79092-R$$9info:eu-repo/grantAgreement/ES/MINECO/DPI2015-65401-C3-1-R$$9info:eu-repo/grantAgreement/ES/MINECO/MAT2015-69967-C3-1
000087566 540__ $$9info:eu-repo/semantics/openAccess$$aby$$uhttp://creativecommons.org/licenses/by/3.0/es/
000087566 590__ $$a3.998$$b2019
000087566 591__ $$aMULTIDISCIPLINARY SCIENCES$$b17 / 71 = 0.239$$c2019$$dQ1$$eT1
000087566 592__ $$a1.341$$b2019
000087566 593__ $$aMultidisciplinary$$c2019$$dQ1
000087566 655_4 $$ainfo:eu-repo/semantics/article$$vinfo:eu-repo/semantics/publishedVersion
000087566 700__ $$aSantos Vizcaíno, Edorta
000087566 700__ $$0(orcid)0000-0001-6139-5905$$aPaz, Laura$$uUniversidad de Zaragoza
000087566 700__ $$aGallardo-Moreno, Amparo María
000087566 700__ $$aOrive, Gorka
000087566 700__ $$aHernández, Rosa María
000087566 700__ $$0(orcid)0000-0001-8741-6452$$aDoblaré, Manuel$$uUniversidad de Zaragoza
000087566 700__ $$aGonzalez-Martin, Maria Luisa
000087566 700__ $$0(orcid)0000-0001-5376-4440$$aFernández, Luis Jose$$uUniversidad de Zaragoza
000087566 700__ $$aPedraz, Jose Luis
000087566 700__ $$0(orcid)0000-0003-2410-5678$$aOchoa, Ignacio$$uUniversidad de Zaragoza
000087566 7102_ $$11003$$2443$$aUniversidad de Zaragoza$$bDpto. Anatom.Histolog.Humanas$$cArea Histología
000087566 7102_ $$15004$$2605$$aUniversidad de Zaragoza$$bDpto. Ingeniería Mecánica$$cÁrea Mec.Med.Cont. y Teor.Est.
000087566 773__ $$g9 (2019), 20112 [14 pp.]$$pSci. rep. (Nat. Publ. Group)$$tScientific reports (Nature Publishing Group)$$x2045-2322
000087566 8564_ $$s8539523$$uhttps://zaguan.unizar.es/record/87566/files/texto_completo.pdf$$yVersión publicada
000087566 8564_ $$s101637$$uhttps://zaguan.unizar.es/record/87566/files/texto_completo.jpg?subformat=icon$$xicon$$yVersión publicada
000087566 909CO $$ooai:zaguan.unizar.es:87566$$particulos$$pdriver
000087566 951__ $$a2023-09-21-13:30:04
000087566 980__ $$aARTICLE