87566 20230921135430.0 doi 10.1038/s41598-019-56547-z sideral 115459 ART-2019-115459 eng Virumbrales Muñoz, María (orcid)0000-0003-3660-8651 Force spectroscopy-based simultaneous topographical and mechanical characterization to study polymer-to-polymer interactions in coated alginate microspheres 2019 Access copy available to the general public Unrestricted Cell-laden hydrogel microspheres have shown encouraging outcomes in the fields of drug delivery, tissue engineering or regenerative medicine. Beyond the classical single coating with polycations, many other different coating designs have been reported with the aim of improving mechanical properties and in vivo performance of the microspheres. Among the most common strategies are the inclusion of additional polycation coatings and the covalent bonding of the semi-permeable membranes with biocompatible crosslinkers such as genipin. However, it remains challenging to characterize the effects of the interactions between the polycations and the hydrogel microspheres over time in vitro. Here we use a force spectroscopy-based simultaneous topographical and mechanical characterization to study polymer-to-polymer interactions in alginate microspheres with different coating designs, maintaining the hydrogels in liquid. In addition to classical topography parameters, we explored, for the first time, the evolution of peak/valley features along the z axis via thresholding analysis and the cross-correlation between topography and stiffness profiles with resolution down to tens of nanometers. Thus, we demonstrated the importance of genipin crosslinking to avoid membrane detachment in alginate microspheres with double polycation coatings. Overall, this methodology could improve hydrogel design rationale and expedite in vitro characterization, therefore facilitating clinical translation of hydrogel-based technologies. info:eu-repo/grantAgreement/ES/DGA/T24-17R info:eu-repo/grantAgreement/EC/H2020/783132/EU/A pilot line for the next generation of smart catheters and implants/POSITION-II This project has received funding from the European Union’s Horizon 2020 research and innovation program under grant agreement No H2020 783132-POSITION-II info:eu-repo/grantAgreement/ES/MICINN/MTM2012-31073 info:eu-repo/grantAgreement/ES/MINECO/BIO2016-79092-R info:eu-repo/grantAgreement/ES/MINECO/DPI2015-65401-C3-1-R info:eu-repo/grantAgreement/ES/MINECO/MAT2015-69967-C3-1 info:eu-repo/semantics/openAccess by http://creativecommons.org/licenses/by/3.0/es/ 3.998 2019 MULTIDISCIPLINARY SCIENCES 17 / 71 = 0.239 2019 Q1 T1 1.341 2019 Multidisciplinary 2019 Q1 info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion Santos Vizcaíno, Edorta Paz, Laura Universidad de Zaragoza (orcid)0000-0001-6139-5905 Gallardo-Moreno, Amparo María Orive, Gorka Hernández, Rosa María Doblaré, Manuel Universidad de Zaragoza (orcid)0000-0001-8741-6452 Gonzalez-Martin, Maria Luisa Fernández, Luis Jose Universidad de Zaragoza (orcid)0000-0001-5376-4440 Pedraz, Jose Luis Ochoa, Ignacio Universidad de Zaragoza (orcid)0000-0003-2410-5678 1003 443 Universidad de Zaragoza Dpto. Anatom.Histolog.Humanas Area Histología 5004 605 Universidad de Zaragoza Dpto. Ingeniería Mecánica Área Mec.Med.Cont. y Teor.Est. 9 (2019), 20112 [14 pp.] Sci. rep. (Nat. Publ. Group) Scientific reports (Nature Publishing Group) 2045-2322 8539523 http://zaguan.unizar.es/record/87566/files/texto_completo.pdf Versión publicada 101637 http://zaguan.unizar.es/record/87566/files/texto_completo.jpg?subformat=icon icon Versión publicada oai:zaguan.unizar.es:87566 articulos driver 2023-09-21-13:30:04 ARTICLE