000087586 001__ 87586
000087586 005__ 20230921135431.0
000087586 0247_ $$2doi$$a10.3390/biom10010016
000087586 0248_ $$2sideral$$a115787
000087586 037__ $$aART-2019-115787
000087586 041__ $$aeng
000087586 100__ $$aDuran-Sanchon, S.
000087586 245__ $$aValidation of miR-1228-3p as Housekeeping for MicroRNA Analysis in Liquid Biopsies from Colorectal Cancer Patients
000087586 260__ $$c2019
000087586 5060_ $$aAccess copy available to the general public$$fUnrestricted
000087586 5203_ $$aBACKGROUND: Circulating microRNA (miRNA) analysis is a growing research field. However, it usually requires an endogenous control or housekeeping (HK) in order to normalize expression of specific miRNAs throughout different samples. Unfortunately, no adequate HK for circulating miRNA analysis is still known in the colorectal cancer (CRC) context whereas several have been suggested. Hence, our aims were to validate the previously suggested miR-1228-3p as HK for CRC studies, to compare its suitability with the widely used miR-16-5p, and to evaluate the influence of hemolysis on both miRNAs. METHODS: We analyzed by quantitative reverse-transcription polymerase chain reaction (qRT-PCR) the expression of miR-1228-3p, miR-16-5p and the spike-in cel-miR-39 in a set of 297 plasmas (92 CRC, 101 advanced adenomas -AA-, and 100 controls) and 213 serum samples (59 CRC, 74 AA and 80 controls). We also analyzed both miRNAs depending on the hemolysis degree in 7 plasmas and 31 serums. RESULTS: Levels of miR-1228-3p and miR-16-5p did not show significant differences between groups although miR-16-5p exhibited more variability in plasma and serum samples. Importantly, the combination of cel-miR-39 and miR-1228-3p was the most stable one. Moreover, we observed that miR-16-5p was significantly influenced by hemolysis in contrast with miR-1228-3p that exhibited no correlation with this confounding factor in both biofluids. CONCLUSION: MiR-1228-3p has been validated as an adequate endogenous control for circulating miRNA analysis in CRC and AA liquid biopsies.
000087586 536__ $$9info:eu-repo/grantAgreement/ES/ISCIII/PI17-01003$$9info:eu-repo/grantAgreement/ES/MINECO/RTC-2015-3850-1$$9info:eu-repo/grantAgreement/ES/MINECO/SAF2014-54453-R
000087586 540__ $$9info:eu-repo/semantics/openAccess$$aby$$uhttp://creativecommons.org/licenses/by/3.0/es/
000087586 590__ $$a4.082$$b2019
000087586 591__ $$aBIOCHEMISTRY & MOLECULAR BIOLOGY$$b98 / 296 = 0.331$$c2019$$dQ2$$eT2
000087586 592__ $$a1.614$$b2019
000087586 593__ $$aBiochemistry$$c2019$$dQ1
000087586 593__ $$aMolecular Biology$$c2019$$dQ2
000087586 655_4 $$ainfo:eu-repo/semantics/article$$vinfo:eu-repo/semantics/publishedVersion
000087586 700__ $$aVila-Navarro, E.
000087586 700__ $$aMarcuello, M.
000087586 700__ $$aLozano, J.J.
000087586 700__ $$aMuñoz, J.
000087586 700__ $$aCubiella, J.
000087586 700__ $$aDiez, M.S.
000087586 700__ $$aBujanda, L.
000087586 700__ $$0(orcid)0000-0001-5932-2889$$aLanas, A.$$uUniversidad de Zaragoza
000087586 700__ $$aJover, R.
000087586 700__ $$aHernández, V.
000087586 700__ $$aQuintero, E.
000087586 700__ $$aHerreros-Villanueva, M.
000087586 700__ $$aMartín, A.C.
000087586 700__ $$aPérez-Palacios, R.
000087586 700__ $$aArroyo, R.
000087586 700__ $$aCastells, A.
000087586 700__ $$aGironella, M.
000087586 7102_ $$11007$$2610$$aUniversidad de Zaragoza$$bDpto. Medicina, Psiqu. y Derm.$$cArea Medicina
000087586 773__ $$g10, 1 (2019), 16 [11 pp.]$$tBiomolecules$$x2218-273X
000087586 8564_ $$s734131$$uhttps://zaguan.unizar.es/record/87586/files/texto_completo.pdf$$yVersión publicada
000087586 8564_ $$s51800$$uhttps://zaguan.unizar.es/record/87586/files/texto_completo.jpg?subformat=icon$$xicon$$yVersión publicada
000087586 909CO $$ooai:zaguan.unizar.es:87586$$particulos$$pdriver
000087586 951__ $$a2023-09-21-13:30:09
000087586 980__ $$aARTICLE