000087620 001__ 87620
000087620 005__ 20210301081640.0
000087620 0247_ $$2doi$$a10.2478/s11686-019-00113-0
000087620 0248_ $$2sideral$$a115838
000087620 037__ $$aART-2019-115838
000087620 041__ $$aeng
000087620 100__ $$aDebbou-Iouknane, N.
000087620 245__ $$aIn Vitro Anticoccidial Activity of Olive Pulp (Olea europaea L. var. Chemlal) Extract Against Eimeria Oocysts in Broiler Chickens
000087620 260__ $$c2019
000087620 5060_ $$aAccess copy available to the general public$$fUnrestricted
000087620 5203_ $$aAim: The objective of the present study was to investigate in vitro anticoccidial effect of olive pulp (Olea europaea L var. Chemlal) extract on the destruction of Eimeria spp. oocysts isolated from infected chickens naturally. Materials and methods: The olive pulp (OP) powder was stirred manually in aqueous ethanol in preparation for extraction using the microwave-assisted extraction system. The identification of the phenolic compounds was obtained by ultra-high-performance liquid chromatography–mass spectrometry with electrospray ionisation (HPLC–ESI–MS). The treatment of Eimeria oocyst with OP extract and standard compounds (quercetin and oleuropein) leads to their lysis as shown by the release of substances absorbing at 273 nm. Results: Our results showed that the maximum number of reduced oocysts was recorded after 8 h of incubation of optimum OP extract, quercetin and oleuropein for different periods of time. Also, the number of Eimeria oocysts decreased considerably with increase concentrations after adding the optimum of OP extract in concentration ranging from 0.023 to 0.371 mg/ml. Positive correlation between the optimum OP extract concentrations and the number of Eimeria oocysts reduced was R2 = 0.959. From this in vitro experiment, it can be concluded that the OP extract possesses an anti-Eimeria spp activity. Conclusion: To our knowledge, this is the first time that quercetin and oleuropein were tested to evaluate their anticoccidial activity. The findings of this study showed that phenolic compound of OP extract tested separately possesses anti-Eimeria spp. effect. Further studies should be carried out to test its in vivo efficacy of the OP bioactive compounds in broiler chickens.
000087620 540__ $$9info:eu-repo/semantics/openAccess$$aby$$uhttp://creativecommons.org/licenses/by/3.0/es/
000087620 590__ $$a1.019$$b2019
000087620 591__ $$aPARASITOLOGY$$b34 / 39 = 0.872$$c2019$$dQ4$$eT3
000087620 592__ $$a0.467$$b2019
000087620 593__ $$aParasitology$$c2019$$dQ3
000087620 655_4 $$ainfo:eu-repo/semantics/article$$vinfo:eu-repo/semantics/publishedVersion
000087620 700__ $$0(orcid)0000-0003-2685-5739$$aNerín, C.$$uUniversidad de Zaragoza
000087620 700__ $$aAmrane, M.
000087620 700__ $$aGhemghar, M.
000087620 700__ $$aMadani, K.
000087620 700__ $$aAyad, A.
000087620 7102_ $$12009$$2750$$aUniversidad de Zaragoza$$bDpto. Química Analítica$$cÁrea Química Analítica
000087620 773__ $$g64, 4 (2019), 887-897$$pActa Parasitol.$$tACTA PARASITOLOGICA$$x1230-2821
000087620 8564_ $$s829540$$uhttps://zaguan.unizar.es/record/87620/files/texto_completo.pdf$$yVersión publicada
000087620 8564_ $$s102830$$uhttps://zaguan.unizar.es/record/87620/files/texto_completo.jpg?subformat=icon$$xicon$$yVersión publicada
000087620 909CO $$ooai:zaguan.unizar.es:87620$$particulos$$pdriver
000087620 951__ $$a2021-03-01-08:01:56
000087620 980__ $$aARTICLE