87746 20200716101551.0 doi 10.3390/ijms20225557 sideral 115871 ART-2019-115871 eng Lusinska, Joanna Comparatively barcoded chromosomes of brachypodium perennials tell the story of their karyotype structure and evolution 2019 Access copy available to the general public Unrestricted The Brachypodium genus is an informative model system for studying grass karyotype organization. Previous studies of a limited number of species and reference chromosomes have not provided a comprehensive picture of the enigmatic phylogenetic relationships in the genus. Comparative chromosome barcoding, which enables the reconstruction of the evolutionary history of individual chromosomes and their segments, allowed us to infer the relationships between putative ancestral karyotypes of extinct species and extant karyotypes of current species. We used over 80 chromosome-specific BAC (bacterial artificial chromosome) clones derived from five reference chromosomes of B. distachyon as probes against the karyotypes of twelve accessions representing five diploid and polyploid Brachypodium perennials. The results showed that descending dysploidy is common in Brachypodium and occurs primarily via nested chromosome fusions. Brachypodium distachyon was rejected as a putative ancestor for allotetraploid perennials and B. stacei for B. mexicanum. We propose two alternative models of perennial polyploid evolution involving either the incorporation of a putative x = 5 ancestral karyotype with different descending dysploidy patterns compared to B. distachyon chromosomes or hybridization of two x = 9 ancestors followed by genome doubling and descending dysploidy. Details of the karyotype structure and evolution in several Brachypodium perennials are revealed for the first time. info:eu-repo/semantics/openAccess by http://creativecommons.org/licenses/by/3.0/es/ 4.556 2019 CHEMISTRY, MULTIDISCIPLINARY 48 / 177 = 0.271 2019 Q2 T1 BIOCHEMISTRY & MOLECULAR BIOLOGY 74 / 297 = 0.249 2019 Q1 T1 1.317 2019 Medicine (miscellaneous) 2019 Q1 Physical and Theoretical Chemistry 2019 Q1 Computer Science Applications 2019 Q1 Inorganic Chemistry 2019 Q1 Spectroscopy 2019 Q1 Organic Chemistry 2019 Q1 Molecular Biology 2019 Q2 Catalysis 2019 Q2 info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion Betekhtin, Alexander (orcid)0000-0002-7734-8481 Lopez-Alvarez, Diana (orcid)0000-0001-7793-5259 Catalan, Pilar Universidad de Zaragoza Jenkins, Glyn Wolny, Elzbieta Hasterok, Robert 5011 063 Universidad de Zaragoza Dpto. CC.Agrar.y Medio Natural Área Botánica 20, 22 (2019), 5557 [19 pp.] Int. j. mol. sci. International Journal of Molecular Sciences 1661-6596 3463097 http://zaguan.unizar.es/record/87746/files/texto_completo.pdf Versión publicada 481578 http://zaguan.unizar.es/record/87746/files/texto_completo.jpg?subformat=icon icon Versión publicada oai:zaguan.unizar.es:87746 articulos driver 2020-07-16-09:46:30 ARTICLE