000087826 001__ 87826
000087826 005__ 20200716101421.0
000087826 0247_ $$2doi$$a10.1016/j.jevs.2019.02.010
000087826 0248_ $$2sideral$$a111167
000087826 037__ $$aART-2019-111167
000087826 041__ $$aeng
000087826 100__ $$aÁlvarez, C.
000087826 245__ $$aEffect of Mare Colostrum in Extenders for Freezing Stallion Semen
000087826 260__ $$c2019
000087826 5060_ $$aAccess copy available to the general public$$fUnrestricted
000087826 5203_ $$aThis study aimed to evaluate the addition of mare colostrum in stallion freezing extenders to improve sperm quality. First, colostrum samples were collected from four mares after the foal''s birth and their composition was determined. Ejaculates were collected from nine fertile stallions. Sperm samples were pooled, diluted, and cryopreserved into three experimental extender groups: Lactose-based extender supplemented with mare colostrum (20%), lactose-based extender supplemented with egg yolk (20%), and BotuCrio. The quality of the post-thaw semen samples were evaluated assessing sperm motility by means of computer-assisted analysis, viability by SYBR-14 and propidium iodine (PI) stain, acrosome integrity by fluorescein isothiocyanate and peanut agglutinine (FITC-PNA) and PI stain, plasma membrane functionality by hypo-osmotic swelling (HOS) test, and DNA denaturation by acridine orange (AO) test. There were no significant differences in the percentages of total motility, acrosome integrity, and DNA fragmentation among the extenders after thawing. Kinematics parameters showed significantly higher values in BotuCrio than in lactose extenders (P < .05). BotuCrio and lactose colostrum extender yielded significantly better rates for HOS-test, linearity, straightness, and wobble than egg-yolk extender (P < .05). However, in relation to sperm viability, lactose egg yolk extender showed significantly better results in comparison to the others seminal experimental media (P < .05). In conclusion, the incorporation of mare colostrum into cryopreservation media protected the sperm against cold-shock; therefore, it may be a good cryoprotectant agent alternative in extenders for freezing stallion semen.
000087826 536__ $$9info:eu-repo/grantAgreement/ES/DGA/Research Groups-European Social Found
000087826 540__ $$9info:eu-repo/semantics/openAccess$$aAll rights reserved$$uhttp://www.europeana.eu/rights/rr-f/
000087826 590__ $$a1.1$$b2019
000087826 592__ $$a0.424$$b2019
000087826 591__ $$aVETERINARY SCIENCES$$b75 / 142 = 0.528$$c2019$$dQ3$$eT2
000087826 593__ $$aEquine$$c2019$$dQ3
000087826 655_4 $$ainfo:eu-repo/semantics/article$$vinfo:eu-repo/semantics/acceptedVersion
000087826 700__ $$0(orcid)0000-0002-7857-9732$$aLuño, V.$$uUniversidad de Zaragoza
000087826 700__ $$0(orcid)0000-0002-1611-6337$$aGonzález, N.$$uUniversidad de Zaragoza
000087826 700__ $$aGuerra, P.
000087826 700__ $$0(orcid)0000-0002-0797-1841$$aGil, L.$$uUniversidad de Zaragoza
000087826 7102_ $$11009$$2617$$aUniversidad de Zaragoza$$bDpto. Patología Animal$$cÁrea Medicina y Cirugía Animal
000087826 773__ $$g77 (2019), 23-27$$pJ. equine vet. sci.$$tJOURNAL OF EQUINE VETERINARY SCIENCE$$x0737-0806
000087826 8564_ $$s335947$$uhttps://zaguan.unizar.es/record/87826/files/texto_completo.pdf$$yPostprint
000087826 8564_ $$s177558$$uhttps://zaguan.unizar.es/record/87826/files/texto_completo.jpg?subformat=icon$$xicon$$yPostprint
000087826 909CO $$ooai:zaguan.unizar.es:87826$$particulos$$pdriver
000087826 951__ $$a2020-07-16-08:41:10
000087826 980__ $$aARTICLE