88170 20200225105134.0 doi 10.3390/agriengineering2010005 sideral 116248 ART-2020-116248 eng Matei, Petruta Mihaela In vitro antifungal activity of chitosan-polyphenol conjugates against Phytophthora cinnamomi 2020 Access copy available to the general public Unrestricted Phytophthora cinnamomi is responsible for radical rot in a wide range of hosts, resulting in large economic and ecological losses worldwide. In Spain, it is responsible for diseases such as the oak decline or the chestnut blight. In this study, different polyphenol-stevioside inclusion compounds dispersed in a hydroalcoholic solution of chitosan oligomers have been investigated, with a view to their application as natural bioactive complexes to replace conventional systemic fungicides against this fungus. The polyphenols tested in vitro were curcumin, ferulic acid, gallic acid and silymarin. Three concentrations (125, 250 and 500 µg·mL-1) were assayed, with and without silver nanoparticles (AgNPs), and notable differences were found in the inhibition of mycelium growth, with EC50 and EC90 values ranging from 171 to 373.6 µg·mL-1, and from 446.2 to 963.7 µg·mL-1, respectively. The results obtained showed that the addition of AgNPs, despite their antimicrobial activity, did not always lead to synergies. In the case of P. cinnamomi, an unexpected antagonistic behavior was found for two of the polyphenols (curcumin and silymarin), while an additive behavior for ferulic acid and a synergistic behavior for gallic acid were attained. In view of their inhibitory power, the preparations based on ferulic acid with AgNPs and on silymarin without AgNPs are proposed for applications in crop and forests protection against P. cinnamomi. info:eu-repo/grantAgreement/ES/UZ/UZ2019-TEC-07 info:eu-repo/semantics/openAccess by http://creativecommons.org/licenses/by/3.0/es/ info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion Buzón Durán, Laura Pérez Lebeña, Eduardo Martín Gil, Jesús Iacomi, Beatrice Michaela Ramos Sánchez, M. Carmen (orcid)0000-0003-2713-2786 Martín Ramos, Pablo Universidad de Zaragoza 5011 500 Universidad de Zaragoza Dpto. CC.Agrar.y Medio Natural Area Ingeniería Agroforestal 2, 1 (2020), 72-77 AgriEngineering AgriEngineering 2624-7402 425279 http://zaguan.unizar.es/record/88170/files/texto_completo.pdf Versión publicada 468179 http://zaguan.unizar.es/record/88170/files/texto_completo.jpg?subformat=icon icon Versión publicada oai:zaguan.unizar.es:88170 articulos driver 2020-02-25-10:25:59 ARTICLE